Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

3.1K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
3.1K
CRISPR01:59

CRISPR

46.3K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
46.3K
CRISPR and crRNAs02:53

CRISPR and crRNAs

14.6K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
14.6K
Homologous Recombination02:31

Homologous Recombination

58.8K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
58.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

DNA Cut-Ligation Cyclization Surpasses Jacobson-Stockmayer J-Factor Expectations by over Threefold.

Biomolecules·2026
Same author

Probing the limits of genetic recoding using multi-omics-guided evolution.

Nature communications·2026
Same author

Architectural fragility of gene regulatory networks underlies hematopoietic stem cell aging.

bioRxiv : the preprint server for biology·2026
Same author

Programmable Nucleic Acid Sensing in Human Cells Using Circularizable ssDNA.

Nature communications·2026
Same author

Designing genome editing experiments with EditABLE.

Genome biology·2026
Same author

A platform to design and optimise fluorogenic scFvs for detection of interleukin 33.

Chemical science·2026

Related Experiment Video

Updated: Apr 27, 2026

Genome Editing in Mammalian Cell Lines using CRISPR-Cas
07:56

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

21.5K

Genome editing assessment using CRISPR Genome Analyzer (CRISPR-GA).

Marc Güell1, Luhan Yang2, George M Church2

  • 1Department of Genetics and Biological and Biomedical Sciences Program, Harvard Medical School, Boston, MA 02115 and Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, MA 02138, USA.

Bioinformatics (Oxford, England)
|July 4, 2014
PubMed
Summary

A new computational tool, CRISPR Genome Analyzer, assesses gene editing quality by analyzing next-generation sequencing data. This platform quantifies insertions, deletions, and homologous recombination for improved genome engineering experiments.

More Related Videos

Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors
09:22

Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors

Published on: February 28, 2021

5.0K
CIRCLE-Seq for Interrogation of Off-Target Gene Editing
08:23

CIRCLE-Seq for Interrogation of Off-Target Gene Editing

Published on: November 1, 2024

1.7K

Related Experiment Videos

Last Updated: Apr 27, 2026

Genome Editing in Mammalian Cell Lines using CRISPR-Cas
07:56

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

21.5K
Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors
09:22

Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors

Published on: February 28, 2021

5.0K
CIRCLE-Seq for Interrogation of Off-Target Gene Editing
08:23

CIRCLE-Seq for Interrogation of Off-Target Gene Editing

Published on: November 1, 2024

1.7K

Area of Science:

  • Genomics
  • Bioinformatics
  • Molecular Biology

Background:

  • CRISPR-based technologies offer revolutionary genome engineering capabilities.
  • Current methods for assessing gene editing outcomes lack sensitivity and flexibility.
  • A need exists for robust computational tools for genome editing assessment.

Purpose of the Study:

  • To introduce CRISPR Genome Analyzer (CRISPR-GA), a novel computational platform for evaluating genome editing experiments.
  • To provide a user-friendly tool for the quantitative and qualitative analysis of gene editing outcomes.
  • To address the limitations of existing experimental and computational assessment methods.

Main Methods:

  • CRISPR-GA analyzes next-generation sequencing data.
  • The platform quantifies and characterizes insertions, deletions, and homologous recombination events.
  • It maps sequencing reads, estimates indel frequencies, and calculates allele replacement efficiency.

Main Results:

  • CRISPR-GA provides a comprehensive report for selected genomic loci.
  • The report includes quantification of the edited site and detailed analysis of detected alterations.
  • The platform integrates various data points to offer a holistic view of editing quality.

Conclusions:

  • CRISPR Genome Analyzer offers a sensitive, flexible, and user-friendly solution for genome editing assessment.
  • The tool facilitates rigorous post-editing analysis, crucial for advancing gene editing applications.
  • CRISPR-GA supports basic science, synthetic biology, and gene therapy by improving experimental validation.