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Updated: Apr 27, 2026
A Polyaniline-based Sensor of Nucleic Acids
Published on: November 1, 2016
Highly selective and sensitive nucleic acid detection based on polysaccharide-functionalized silver nanoparticles
Jing-Kun Yan1, Hai-Le Ma2, Pan-Fu Cai2
1The Key Laboratory of Carbohydrate Chemistry & Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, Jiangsu, China; School of Food & Biological Engineering, Jiangsu University, Zhenjiang 212013, China; Department of Applied Biology & Chemical Technology, State Key Laboratory of Chinese Medicine and Molecular Pharmacology in Shenzhen, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong.
Abstract:
Polysaccharide-functionalized silver nanoparticles (Oc-AgNPs) with a mean diameter of 15 nm were utilized as a novel and effective fluorescence-sensing platform for nucleic acid detection. Tests on the oligonucleotide sequences associated with the human immunodeficiency virus as a model system showed that the Oc-AgNPs effectively absorbed and quenched dye-labeled single-stranded DNA through strong hydrogen bonding interactions and slight electrostatic attractive interactions. The proposed system efficiently differentiated between complementary and mismatched nucleic acid sequences with high selectivity and good reproducibility at room temperature.
