Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

2.6K
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
2.6K
Adsorption Isotherms I01:29

Adsorption Isotherms I

227
Adsorption isotherms are mathematical models that describe how molecules in a gas or liquid phase interact with surfaces. Two of the most common isotherm models are the Langmuir and Freundlich isotherms, which relate to Type I monolayer chemisorption. The Langmuir model is based on four key assumptions:• Adsorption cannot exceed monolayer coverage.• All surface sites are equivalent.• Molecules adsorb only at vacant sites.• There are no interactions between adsorbed...
227
Adsorption of Gases on Solids01:28

Adsorption of Gases on Solids

274
Adsorption is a process where molecules, known as the adsorbates, accumulate on a surface, which is referred to as the adsorbent or substrate. Occurring at the solid-gas interface, this phenomenon is crucial in various scientific and industrial contexts. The reverse of adsorption is desorption.Two types of adsorptions exist: physical (physisorption) and chemical (chemisorption). Physisorption involves gas molecules held to the solid's surface by relatively weak intermolecular van der Waals...
274
Adsorption Isotherms II01:25

Adsorption Isotherms II

136
Brunauer, Emmett, and Teller (BET) introduced a theory in 1938 that modified Langmuir's assumptions to explain multilayer physical adsorption. This theory is applicable to Type II isotherms and provides a more realistic picture of adsorption processes. The BET theory assumes a uniform solid surface with localized adsorption sites, where adsorption at one site doesn't affect adsorption at neighboring sites. This theory also allows for the possibility of additional molecules being adsorbed on top...
136

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Scatter-Free UV-Visible Spectroscopy for Accurate and Precise RNA Quantification in Complex RNA Nanoparticle Formulations.

Analytical chemistry·2025
Same author

Rapid and Accurate Quantification of RNA in Lipid Nanoparticles by Scatter-Free UV/Visible Spectroscopy.

Nano letters·2025
Same author

Morphological Control of Y6 Thin Films Reveals Charge Transfer Is Facilitated by Co-facial Interactions.

The journal of physical chemistry letters·2025
Same author

Surface-enhanced Raman spectroscopy: a half-century historical perspective.

Chemical Society reviews·2024
Same author

Forward-Scattering and Multiple-Scattering Sources of Errors in UV-Visible Spectroscopy of Microspheres.

Analytical chemistry·2024
Same author

Enhancement Factors: A Central Concept during 50 Years of Surface-Enhanced Raman Spectroscopy.

ACS nano·2024
Same journal

Gas-Responsive Metal-Organic Frameworks for Adaptive Thermal Energy Storage with Tunable Charge-Discharge Temperatures.

Journal of the American Chemical Society·2026
Same journal

Engineering a Thiamine-Dependent Benzoylformate Decarboxylase for Stereodivergent Radical C(sp<sup>3</sup>)-C(sp<sup>3</sup>) Bond Formation.

Journal of the American Chemical Society·2026
Same journal

Accelerated Directional Proton-Coupled Electron Transfer Enabled by Intrinsic Dipole Field in Biomimetic α-Helical Structure.

Journal of the American Chemical Society·2026
Same journal

Alternating Current-Driven Hydrogen Isotope Labeling of Aliphatic Amines Using 1,3-Propanedithiol as an Efficient Hydrogen Atom Transfer Reagent.

Journal of the American Chemical Society·2026
Same journal

Two-Dimensional van der Waals Polar Metal MoOBr<sub>2</sub>.

Journal of the American Chemical Society·2026
Same journal

Negatively Curved Chiral Bilayer Nanographene.

Journal of the American Chemical Society·2026
See all related articles

Related Experiment Video

Updated: Apr 26, 2026

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
11:44

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Published on: March 20, 2015

24.5K

Competition between molecular adsorption and diffusion: dramatic consequences for SERS in colloidal solutions.

Brendan L Darby1, Eric C Le Ru

  • 1The MacDiarmid Institute for Advanced Materials and Nanotechnology School of Chemical and Physical Sciences, Victoria University of Wellington , P.O. Box 600, Wellington 6140, New Zealand.

Journal of the American Chemical Society
|July 22, 2014
PubMed
Summary
This summary is machine-generated.

Dilution methods significantly impact surface-adsorbing species analysis. Fast adsorption with large dilutions causes uneven surface coverage, affecting surface-enhanced Raman spectroscopy (SERS) signals and aggregation. A simple half-half dilution is recommended.

More Related Videos

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging
09:46

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging

Published on: April 28, 2022

3.6K
A Filter-based Surface Enhanced Raman Spectroscopic Assay for Rapid Detection of Chemical Contaminants
08:13

A Filter-based Surface Enhanced Raman Spectroscopic Assay for Rapid Detection of Chemical Contaminants

Published on: February 19, 2016

8.7K

Related Experiment Videos

Last Updated: Apr 26, 2026

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
11:44

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Published on: March 20, 2015

24.5K
Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging
09:46

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging

Published on: April 28, 2022

3.6K
A Filter-based Surface Enhanced Raman Spectroscopic Assay for Rapid Detection of Chemical Contaminants
08:13

A Filter-based Surface Enhanced Raman Spectroscopic Assay for Rapid Detection of Chemical Contaminants

Published on: February 19, 2016

8.7K

Area of Science:

  • Analytical Chemistry
  • Spectroscopy
  • Materials Science

Background:

  • Analyte distribution is critical in sample preparation for surface-adsorbing species.
  • Surface-enhanced Raman spectroscopy (SERS) often employs large dilution factors.
  • The interplay between analyte adsorption and diffusion is frequently overlooked.

Purpose of the Study:

  • To investigate the impact of dilution methods on analyte distribution during sample preparation.
  • To demonstrate how dilution affects surface coverage and aggregation in colloidal solutions.
  • To highlight the implications for surface-enhanced Raman spectroscopy (SERS) signal uniformity and statistics.

Main Methods:

  • Comparative analysis of different dilution techniques for surface-adsorbing analytes.
  • Utilizing surface-enhanced Raman spectroscopy (SERS) to probe analyte distribution and aggregation.
  • Investigating the influence of dilution on colloidal solution properties and SERS signal intensity.

Main Results:

  • Large dilution factors in the presence of fast analyte adsorption lead to extreme nonuniformity in surface coverage.
  • This nonuniformity directly impacts the aggregation state of colloidal solutions and the resulting SERS signal.
  • Significant differences in SERS signals were observed between samples diluted using different methods, despite apparent equivalence.

Conclusions:

  • The dilution step is a critical factor in experiments involving surface-adsorbing species.
  • Surface-enhanced Raman spectroscopy (SERS) can effectively reveal nonuniform analyte distribution caused by dilution.
  • Adopting a simple half-half dilution procedure is proposed as a standard method to mitigate these effects and ensure reliable SERS analysis.