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CELLCOUNTER: novel open-source software for counting cell migration and invasion in vitro
Xiaoni Li1, Hongshun Yang2, Hailiang Huang3
1Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, USTC, Hefei, Anhui, China.
Biomed Research International
|July 24, 2014
Summary
Manual cell counting in migration assays is tedious. We developed CELLCOUNTER, an automated application to accurately quantify cell motility, improving experimental efficiency and data reliability for in vitro cell migration studies.
Area of Science:
- Cell Biology
- Biotechnology
Background:
- Transwell Boyden chamber assays are standard for in vitro cell motility assessment.
- Manual cell counting in these assays is time-consuming and prone to errors.
- Accurate quantification of cell migration and invasion is crucial for biological research.
Purpose of the Study:
- To develop an automated application for cell counting in Transwell assays.
- To improve the efficiency and reliability of cell motility data acquisition.
- To provide a user-friendly tool for analyzing cell migration and invasion.
Main Methods:
- Development of the CELLCOUNTER application with a graphical user interface.
- Utilizing image recognition for automated cell counting.
- Implementing batch processing and manual data curation features.
Main Results:
- CELLCOUNTER accurately recognizes and counts cells passing through filter membranes.
- The application streamlines the laborious manual counting process.
- Automated counting enhances the reliability of cell motility data.
Conclusions:
- CELLCOUNTER offers a significant improvement over manual cell counting methods.
- The application aids in streamlining experimental workflows for cell migration assays.
- Automated cell counting with CELLCOUNTER enhances data quality and reproducibility.
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