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In-vitro Mutagenesis

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

Mouse Models of Cancer Study

Mouse Models of Cancer Study

Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...

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Related Experiment Video

Updated: Apr 26, 2026

Lineage Tracing of Inducible Fluorescently-Labeled Stem Cells in the Adult Mouse Brain

Lineage Tracing of Inducible Fluorescently-Labeled Stem Cells in the Adult Mouse Brain

Published on: May 20, 2022

Generating mouse lines for lineage tracing and knockout studies.

Petra Kraus¹, V Sivakamasundari, Xing Xing

¹Department of Biology, Clarkson University, Potsdam, 13699-5808, USA.

Methods in Molecular Biology (Clifton, N.J.)

|July 28, 2014

Summary

This summary is machine-generated.

Detailed protocols enable precise gene modification in mice using embryonic stem cells. This allows for studying gene function and isolating rare cell types for advanced analysis.

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Generation of Genetically Modified Mice through the Microinjection of Oocytes

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Published on: June 15, 2017

Area of Science:

Biomedical Science
Genetics
Molecular Biology

Background:

Nobel Prize-winning discoveries in 2007 by Capecchi, Evans, and Smithies revolutionized genetic engineering.
Embryonic stem cell technology allows for the generation of genetically engineered animals.

Purpose of the Study:

To describe detailed protocols for gene modification in mice.
To enable the study of gene function through induced mutations.
To facilitate the isolation of specific cell types using fluorescent markers.

Main Methods:

Utilizing principles of gene modification via embryonic stem cells.
Introducing specific mutations to study gene function.
Incorporating fluorescent markers for cell type identification.

Main Results:

Successful modification of genes in mice.
Generation of animals with specific mutations for functional studies.
Isolation of rare wild-type and mutant cell populations.

Conclusions:

Developed protocols provide a robust method for genetic engineering in mice.
Fluorescent marker technology aids in isolating specific cell types for detailed analysis.
These advancements significantly contribute to biomedical research and understanding of gene function.