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Updated: Apr 26, 2026
Aggregate Size Optimization in Microwells for Suspension-based Cardiac Differentiation of Human Pluripotent Stem Cells
Published on: September 25, 2016
Scalable cardiac differentiation of human pluripotent stem cells as microwell-generated, size controlled
Celine L Bauwens1, Mark D Ungrin
1Centre for Commercialization of Regenerative Medicine, 100 College Street, Room 508, Toronto, ON, M5G 1L5, Canada, celine.bauwens@ccrm.ca.
Abstract:
The formation of cells into more physiologically relevant three-dimensional multicellular aggregates is an important technique for the differentiation and manipulation of stem cells and their progeny. As industrial and clinical applications for these cells increase, it will be necessary to execute this procedure in a readily scalable format. We present here a method employing microwells to generate large numbers of human pluripotent stem cell aggregates and control their subsequent differentiation towards a cardiac fate.
