Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

16.0K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
16.0K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.3K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chromatix: a differentiable, GPU-accelerated wave-optics library.

Nature methods·2026
Same author

Correcting curvature in micromirror-based spatial light modulators with a microlens array.

Optics express·2026
Same author

Chromatix: a differentiable, GPU-accelerated wave-optics library.

bioRxiv : the preprint server for biology·2026
Same author

Perturbative Fourier ptychographic microscopy for fast quantitative phase imaging.

Optics express·2025
Same author

Space-time reconstruction for lensless imaging using implicit neural representations.

Optics express·2025
Same author

Sample motion for structured illumination fluorescence microscopy.

Optics letters·2025
Same journal

Generalizable framework for multi-site bone density prediction using non-dominant wrist optical biomarkers.

Biomedical optics express·2026
Same journal

Erratum: Review of dynamic optical coherence tomography for intracellular motility [Invited]: errata.

Biomedical optics express·2026
Same journal

Digital-micromirror-device-based illumination strategies for background suppression in single-molecule localization microscopy.

Biomedical optics express·2026
Same journal

Synergistic combination of convective self-assembly and hollow core fiber for sensitive SERS detection of glucose molecules.

Biomedical optics express·2026
Same journal

Multimodal diagnostic network integrating infrared and mass spectra for lung cancer.

Biomedical optics express·2026
Same journal

Multimodal Optical Biosensing for Precision Medicine and Healthcare: Introduction to the feature issue.

Biomedical optics express·2026
See all related articles

Related Experiment Video

Updated: Apr 26, 2026

Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

9.4K

Multiplexed coded illumination for Fourier Ptychography with an LED array microscope.

Lei Tian1, Xiao Li1, Kannan Ramchandran1

  • 1Department of Electrical Engineering and Computer Sciences, University of California, Berkeley USA.

Biomedical Optics Express
|July 30, 2014
PubMed
Summary
This summary is machine-generated.

Fourier Ptychography uses multiplexed illumination to create gigapixel images faster. This computational microscopy technique significantly reduces image acquisition time and data capture needs for high-resolution phase and amplitude imaging.

Keywords:
(100.5070) Phase retrieval(110.1758) Computational imaging(110.3010) Image reconstruction techniques(170.0180) Microscopy(170.1630) Coded aperture imaging

More Related Videos

Multiplexing Focused Ultrasound Stimulation with Fluorescence Microscopy
08:39

Multiplexing Focused Ultrasound Stimulation with Fluorescence Microscopy

Published on: January 7, 2019

7.9K
Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

8.7K

Related Experiment Videos

Last Updated: Apr 26, 2026

Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

9.4K
Multiplexing Focused Ultrasound Stimulation with Fluorescence Microscopy
08:39

Multiplexing Focused Ultrasound Stimulation with Fluorescence Microscopy

Published on: January 7, 2019

7.9K
Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

8.7K

Area of Science:

  • Computational microscopy
  • Optical imaging
  • Fourier optics

Background:

  • Fourier Ptychography enables gigapixel image reconstruction with high resolution and wide field of view.
  • Traditional Fourier Ptychography requires sequential illumination, leading to long acquisition times.
  • The technique utilizes a programmable LED array for flexible illumination patterning.

Purpose of the Study:

  • To develop a faster and more data-efficient Fourier Ptychography method.
  • To reduce the number of images required for high-quality reconstruction.
  • To demonstrate a multiplexed illumination strategy for computational microscopy.

Main Methods:

  • Implemented a multiplexed illumination strategy using a programmable LED array.
  • Acquired images with multiple randomly selected LEDs illuminated simultaneously.
  • Reconstructed high-resolution phase and amplitude images from multiplexed data.

Main Results:

  • Achieved similar image quality to sequential scanning methods.
  • Reduced the total number of images by an order of magnitude.
  • Significantly decreased both acquisition time and data capture requirements.

Conclusions:

  • Multiplexed illumination is an effective strategy for accelerating Fourier Ptychography.
  • This method offers substantial improvements in efficiency for gigapixel imaging.
  • The technique is experimentally validated on a modified commercial microscope.