Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

ESHRE recommendations on Good Practice in the IVF laboratory†.

Human reproduction (Oxford, England)·2026
Same author

High-avidity TCR signaling induces a distinct KLR-positive exhaustion state in human tumor-infiltrating CD8 T cells associated with immunotherapy response.

bioRxiv : the preprint server for biology·2026
Same author

Fetal Intervention Using an Everolimus-Eluting Stent in HLHS With Intact Atrial Septum.

JACC. Case reports·2026
Same author

Vaginal small-bowel evisceration 15 years post wertheim-hysterectomy: a case report and review of literature.

Frontiers in surgery·2026
Same author

Management of incidental STIC lesions with and without BRCA mutations: a survey of current clinical practice in Austria, Germany, and Switzerland.

Archives of gynecology and obstetrics·2026
Same author

Single-cell analysis highlights the significance of malignant cell IFN/MHC-II for immunotherapy response in head and neck squamous cell carcinoma.

Cell reports. Medicine·2026

Related Experiment Video

Updated: Apr 26, 2026

Mouse Round Spermatid Injection
08:41

Mouse Round Spermatid Injection

Published on: January 26, 2024

1.7K

Some reflections on intracytoplasmic morphologically selected sperm injection.

Thomas Ebner1, Omar Shebl1, Peter Oppelt1

  • 1Department of Gynecological Endocrinology and Kinderwunsch Zentrum, Landes-Frauen-and Kinderklinik and Faculty of Medicine, Johannes Kepler University, Linz, Upper Austria, Austria.

International Journal of Fertility & Sterility
|August 2, 2014
PubMed
Summary
This summary is machine-generated.

Intracytoplasmic sperm injection (ICSI) is unphysiological. Intracytoplasmic morphologically selected sperm injection (IMSI) improves sperm observation but its routine use is not standard, with specific indications for severe male factor and implantation failure.

Keywords:
ChromatinDNAICSISperm HeadVacuole

More Related Videos

Manipulation and In Vitro Maturation of Xenopus laevis Oocytes, Followed by Intracytoplasmic Sperm Injection, to Study Embryonic Development
09:22

Manipulation and In Vitro Maturation of Xenopus laevis Oocytes, Followed by Intracytoplasmic Sperm Injection, to Study Embryonic Development

Published on: February 9, 2015

24.4K
Functional Assessment of Kinesin-7 CENP-E in Spermatocytes Using In Vivo Inhibition, Immunofluorescence and Flow Cytometry
09:41

Functional Assessment of Kinesin-7 CENP-E in Spermatocytes Using In Vivo Inhibition, Immunofluorescence and Flow Cytometry

Published on: December 28, 2021

1.7K

Related Experiment Videos

Last Updated: Apr 26, 2026

Mouse Round Spermatid Injection
08:41

Mouse Round Spermatid Injection

Published on: January 26, 2024

1.7K
Manipulation and In Vitro Maturation of Xenopus laevis Oocytes, Followed by Intracytoplasmic Sperm Injection, to Study Embryonic Development
09:22

Manipulation and In Vitro Maturation of Xenopus laevis Oocytes, Followed by Intracytoplasmic Sperm Injection, to Study Embryonic Development

Published on: February 9, 2015

24.4K
Functional Assessment of Kinesin-7 CENP-E in Spermatocytes Using In Vivo Inhibition, Immunofluorescence and Flow Cytometry
09:41

Functional Assessment of Kinesin-7 CENP-E in Spermatocytes Using In Vivo Inhibition, Immunofluorescence and Flow Cytometry

Published on: December 28, 2021

1.7K

Area of Science:

  • Reproductive Medicine
  • Andrology
  • Assisted Reproductive Technologies

Background:

  • Intracytoplasmic sperm injection (ICSI) is a common but unphysiological assisted reproductive technology (ART).
  • Higher-resolution microscopy techniques like intracytoplasmic morphologically selected sperm injection (IMSI) have been developed to enhance sperm observation.
  • Sperm vacuoles remain a subject of ongoing research regarding their prevalence, origin, and clinical impact.

Purpose of the Study:

  • To review the current literature on sperm vacuoles and the application of motile sperm organelle morphology examination (MSOME) and IMSI.
  • To clarify the clinical significance and indications for IMSI in the context of male infertility.

Main Methods:

  • A literature search of the PubMed database was conducted from 2000 onwards.
  • Keywords used included IMSI, MSOME, and sperm vacuole.
  • The review focuses on studies investigating sperm morphology, vacuoles, and their relation to ART outcomes.

Main Results:

  • Despite advancements, key questions regarding sperm vacuoles persist approximately a decade after MSOME and IMSI introduction.
  • Evidence suggests IMSI is not yet a routine ART procedure.
  • Confirmed indications for IMSI are limited to recurrent implantation failure after ICSI and severe male factor infertility.

Conclusions:

  • IMSI offers improved sperm selection but its widespread application requires further validation.
  • Current evidence supports IMSI primarily for specific, severe cases of male infertility and recurrent implantation failure.
  • Further research is needed to fully understand the role and clinical consequences of sperm vacuoles in male fertility and ART success.