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Related Concept Videos

Production of Organic Acids01:25

Production of Organic Acids

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Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...
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Microbes in Food Production01:29

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Microbial fermentation is central to food biotechnology, enhancing flavor, texture, preservation, and stability. Fermentative microorganisms metabolize carbohydrates into organic acids, alcohols, and other metabolites that inhibit spoilage organisms and improve digestibility while contributing distinctive sensory qualities.In baking, amylases naturally present in flour hydrolyze starch into monosaccharides such as glucose, which Saccharomyces cerevisiae ferments anaerobically. Through...
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Fermentation is a crucial anaerobic metabolic process that enables microbes to derive energy from sugar without relying on oxygen or an electron transport chain. This process is fundamental to various biological and industrial applications and is classified based on the metabolic products generated.Role of Pyruvate in FermentationPyruvate and its derivatives serve as key electron acceptors in fermentative pathways. The oxidation of NADH to regenerate NAD+ is essential for the continuation of...
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Microbes in the Production of Fermented Foods01:27

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Lactic acid bacteria (LAB) and molds are instrumental in fermenting plant-based foods to enhance preservation and ensure year-round availability. These microbial processes convert plant carbohydrates into organic acids and other metabolites that inhibit spoilage organisms and contribute to the sensory qualities of the final product.In sauerkraut production, cabbage goes through a microbial succession that starts with cocci such as Leuconostoc mesenteroides. These microbes begin fermentation by...
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Bioreactor Controls-III01:22

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Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
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Pyruvate is the end product of glycolysis, where glucose is oxidized to pyruvate, simultaneously reducing NAD+ to NADH. Two molecules of ATP are also produced by substrate-level phosphorylation.
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Towards lactic acid bacteria-based biorefineries.

Roberto Mazzoli1, Francesca Bosco2, Itzhak Mizrahi3

  • 1Laboratory of Biochemistry: Proteomics and Metabolic Engineering of Prokaryotes, Department of Life Sciences and Systems Biology, University of Torino, Via Accademia Albertina 13, 10123 Torino, Italy.

Biotechnology Advances
|August 5, 2014
PubMed
Summary
This summary is machine-generated.

Lactic acid bacteria (LAB) are promising for biorefineries, converting biomass into biofuels and valuable products like biodegradable plastics. Research explores enhancing LAB strains for efficient fermentation of low-cost feedstocks.

Keywords:
BacteriocinsButanolCelluloseEthanolGABAMannitolMetabolic engineeringPolyhydroxyalkanoatesPolylactide

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Area of Science:

  • Industrial biotechnology
  • Microbial fermentation
  • Sustainable chemistry

Background:

  • Lactic acid bacteria (LAB) are established industrial microorganisms used in food fermentation, biocontrol, and probiotics.
  • LAB possess metabolic capabilities suitable for bioconversion of diverse biomass into valuable chemicals and biofuels.

Purpose of the Study:

  • To provide a comprehensive overview of current and future industrial applications of LAB in biorefineries.
  • To discuss strategies for enhancing LAB metabolic capacity for utilizing cheaper biomass feedstocks.

Main Methods:

  • Literature review of industrial applications and biorefinery potential of LAB.
  • Analysis of LAB fermentation products, including lactic acid, biofuels, and biopolymers.
  • Exploration of strain development strategies for improved substrate utilization.

Main Results:

  • LAB can produce a wide array of high-value products, including lactic acid for biodegradable plastics (polylactides), biofuels (ethanol, butanol), exopolysaccharides, and nutraceuticals.
  • LAB biomass itself can be a valuable product due to its probiotic properties.
  • Developing LAB with broader substrate metabolic capacity is key for cost-effective biomass conversion.

Conclusions:

  • Lactic acid bacteria represent a versatile platform for future biorefineries, enabling sustainable production of chemicals and biofuels from plant biomass and waste streams.
  • Further research into strain engineering and process optimization is crucial for maximizing LAB's contribution to a circular bioeconomy.