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Published on: August 22, 2018
[Study on diversity of protein between Houttuynia cordata plant]
Objective:
To reveal protein diversity between the same batch of fresh Houttuynia cordata in the same GAP base,and to lay the foundation construction for "node metabolic network".
Methods:
Three methods including the Ramagli improved Bradford law, SDS-PAGE gel electrophoresis method and double wavelength thin-layer scanning method were used to study the total protein content diversity, protein species diversity and various kinds of content variability.
Results:
The molecular weight of 53 plant protein mostly concentrated in the range of 6.5-97.2 kDa, the species diversity was not obvious with main performance for banding color shades; The RSD of zero moment (AUCT), first moment (MCRTT) and second moment (VCRTT) in protein electrophoresis banding was 40.92%, 6.01% and 18.57%, respectively.
Conclusion:
There is rich diversity in different Houttuynia cordata plant in the same GAP base, which provides basis for the foundation of subsequent key protease search, "node metabolic network" construction, and study on the Chinese medicine quality stability.
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