Validated spectrofluorimetric method for determination of selected aminoglycosides

Area of Science:

• Analytical Chemistry
• Pharmaceutical Analysis
• Spectrofluorimetry

Background:

• Aminoglycoside antibiotics are crucial in treating bacterial infections.
• Accurate quantification of aminoglycosides in pharmaceutical formulations is essential for quality control.
• Existing analytical methods may lack sensitivity or selectivity for certain aminoglycosides.

Purpose of the Study:

• To develop and validate a novel, sensitive, and selective spectrofluorimetric method.
• To quantify three key aminoglycoside drugs: neomycin sulfate (NEO), tobramycin (TOB), and kanamycin sulfate (KAN).
• To apply the method for the determination of these drugs in various pharmaceutical dosage forms.

Main Methods:

• The method employs the Hantzsch condensation reaction.
• Aminoglycosides react with acetylacetone and formaldehyde at pH 2.7.
• Fluorescent derivatives are measured at excitation/emission wavelengths of 410 nm/471 nm.

Main Results:

• The fluorescence intensity showed a direct proportional relationship with drug concentration.
• Linear ranges were established: 10-60 ng/mL for NEO, 40-100 ng/mL for TOB, and 5-50 ng/mL for KAN.
• The method demonstrated successful application in determining aminoglycosides in pharmaceutical dosage forms.

Conclusions:

• A highly sensitive and selective spectrofluorimetric method was successfully developed.
• The proposed method provides a reliable approach for the quantification of NEO, TOB, and KAN.
• This technique is suitable for routine quality control analysis of aminoglycoside drugs in their dosage forms.