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Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

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Related Experiment Video

Updated: Apr 25, 2026

NanoDrop Microvolume Quantitation of Nucleic Acids

NanoDrop Microvolume Quantitation of Nucleic Acids

Published on: November 22, 2010

Nucleic acid quantification--progress and pitfalls

Hendrik Emons¹, Philippe Corbisier

¹Institute for Reference Materials and Measurements (IRMM), Joint Research Centre, European Commission, Retieseweg 111, 2440, Geel, Belgium, hendrik.emons@ec.europa.eu.

Analytical and Bioanalytical Chemistry

|August 24, 2014

Summary

No abstract available in PubMed .

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