Related Experiment Video
Updated: Apr 25, 2026
A Strategy for Sensitive, Large Scale Quantitative Metabolomics
Published on: May 27, 2014
Minimalistic sample preparation strategies for LC-MS quantification of large molecule biopharmaceuticals: a case
Katherine Wright1, Dawn Dufield
1Biomarker, Biomeasure & Mass Spectrometry Group, Pfizer Worldwide Research and Development, Andover, MA, USA.
Background:
Large molecule biotherapeutics pose a distinctive bioanalytical challenge for LC-MS assay development, particularly when optimizing sample enrichment steps. Alpha-1 antitrypsin (AAT) is used as an example for highlighting large-molecule assay-development strategies.
Results:
Two sensitive and selective LC-MS/MS-based quantification assays were developed. Fit-for-purpose assay qualifications for BAL and serum matrices were performed by assessing sensitivity, precision and accuracy, dilution linearity and interferences.
Conclusion:
Our approach to sample preparation focuses on optimizing the simplest methodology necessary to generate fit-for-purpose bioanalytical assays. To measure AAT protein levels in preclinical species with selectivity and increased assay sensitivity, a minimalistic sample preparation strategy was adopted that included either traditional direct digestion or a more complicated immunoprecipitation enrichment process.
More Related Videos
10:37Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
Published on: November 15, 2017
06:13Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier
Published on: December 9, 2022