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Updated: Apr 25, 2026

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Published on: April 7, 2014

Quantitative phase-contrast imaging through a scattering media.

Rakesh Kumar Singh, Anandraj M Sharma, Bhargab Das

|August 29, 2014

Summary

This summary is machine-generated.

Researchers developed a new method to recover object information hidden by scattering media. This technique uses intensity correlation and digital holography to reconstruct quantitative phase and amplitude data from laser speckle patterns.

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Last Updated: Apr 25, 2026

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Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects

Published on: February 8, 2014

Area of Science:

Optics and Photonics
Image Reconstruction
Wave Phenomena

Background:

Scattering media obscure object details, preventing direct imaging.
Traditional digital holography fails when light passes through diffusers.
Quantitative phase and amplitude recovery is crucial for many imaging applications.

Purpose of the Study:

To present a novel technique for retrieving quantitative phase and amplitude information from objects obscured by scattering media.
To overcome the limitations of direct holographic recording through diffusers.
To enable non-invasive imaging of hidden objects.

Main Methods:

Utilizing two-point intensity correlation measurements.
Applying digital holography principles.
Reconstructing hologram information from laser speckle patterns.

Main Results:

Successfully recovered quantitative phase and amplitude information of objects hidden behind scattering media.
Demonstrated the effectiveness of the proposed technique in overcoming light scattering effects.
Validated the method through digital reconstruction of holograms from speckle fields.

Conclusions:

The developed technique offers a robust solution for imaging through scattering media.
Intensity correlation combined with digital holography enables quantitative phase and amplitude retrieval.
This method has potential applications in various fields requiring imaging through turbid or scattering environments.