Related Experiment Video
Updated: Feb 15, 2026
11:25
FACS-Isolation and Culture of Fibro-Adipogenic Progenitors and Muscle Stem Cells from Unperturbed and Injured Mouse Skeletal Muscle
Published on: June 8, 2022
7.2K
Skeletal muscle stem cells for muscle regeneration
1Department of Cardiac Development and Remodelling, Max-Planck-Institute for Heart and Lung Research, Ludwigstraße 43, 61231, Bad Nauheim, Germany.
Methods in Molecular Biology (Clifton, N.J.)
|September 1, 2014
Summary
Researchers developed a new method to isolate pure satellite cells, the muscle stem cells responsible for tissue regeneration. This technique allows for detailed studies into their autonomous functions in muscle repair.
Area of Science:
- Muscle stem cell biology
- Skeletal muscle regeneration
- Tissue repair mechanisms
Background:
- Adult skeletal muscle has significant regenerative potential.
- Muscle regeneration relies on satellite cells, a rare stem cell population.
- Satellite cells are located between the basal lamina and sarcolemma of muscle fibers.
Purpose of the Study:
- To describe a reliable method for isolating satellite cells.
- To obtain large and pure populations of satellite cells.
- To facilitate studies on the autonomous properties of satellite cells in muscle regeneration.
Main Methods:
- Isolation of satellite cells from skeletal muscle.
- Ensuring purity and yield of isolated cell populations.
- Utilizing the isolated cells for functional studies.
Main Results:
- Successful isolation of large and pure satellite cell populations.
- The method enables in-depth investigation of satellite cell autonomous properties.
- Facilitates understanding of their role in tissue regeneration.
Conclusions:
- The described method is effective for satellite cell isolation.
- This technique supports research into muscle stem cell function.
- Crucial for unraveling the mechanisms of skeletal muscle regeneration.
Related Concept Videos
00:55Skeletal Muscle Anatomy
92.9K
Skeletal muscle is the most abundant type of muscle in the body. Tendons are the connective tissue that attaches skeletal muscle to bones. Skeletal muscles pull on tendons, which in turn pull on bones to carry out voluntary movements.
92.9K
01:48Classification of Skeletal Muscle Fibers
59.6K
Skeletal muscles continuously produce ATP to provide the energy that enables muscle contractions. Skeletal muscle fibers can be categorized into three types based on differences in their contraction speed and how they produce ATP, as well as physical differences related to these factors. Most human muscles contain all three muscle fiber types, albeit in varying proportions.
Slow-Twitch Muscle Fibers
Slow oxidative, muscle fibers appear red due to large numbers of capillaries and high levels of...
Slow-Twitch Muscle Fibers
Slow oxidative, muscle fibers appear red due to large numbers of capillaries and high levels of...
59.6K
01:15Overview of Skeletal Muscle
15.0K
Skeletal muscles are composed of a bundle of muscle fibers and are attached to bones through tendons. Each skeletal muscle fiber is a single muscle cell. The sarcolemma, the plasma membrane of a skeletal muscle cell, consists of a lipid bilayer and glycocalyx that supports muscle fibers. The sarcolemma extends into the muscle cells to form tubular structures called transverse or T-tubules. Each side of the T-tubules consists of a membrane-bound structure called the sarcoplasmic reticulum,...
15.0K
01:29Relaxation of Skeletal Muscles
6.1K
The period of muscle contraction primarily influences the duration of stimulation at the neuromuscular junction (NMJ), the presence of free calcium ions in the sarcoplasm, and the availability of energy or ATP to support contractions.
When an action potential reaches the axon terminal, it depolarizes the membrane and opens voltage-gated sodium channels. Sodium ions enter the cell, further depolarizing the presynaptic membrane. This depolarization causes voltage-gated calcium channels to open....
When an action potential reaches the axon terminal, it depolarizes the membrane and opens voltage-gated sodium channels. Sodium ions enter the cell, further depolarizing the presynaptic membrane. This depolarization causes voltage-gated calcium channels to open....
6.1K
01:28Disorders of the Skeletal Muscle
2.0K
The clinical conditions affecting the skeletal muscle tissue are broadly categorized as musculoskeletal and neuromuscular disorders.
Musculoskeletal disorders
Musculoskeletal disorders involve injuries and conditions affecting the skeletal muscles and associated connective tissues. These disorders can arise from acute biomechanical stresses or chronic overuse and can occur across different age groups. Common injuries include sprains, fractures, and muscular strains, often resulting from...
Musculoskeletal disorders
Musculoskeletal disorders involve injuries and conditions affecting the skeletal muscles and associated connective tissues. These disorders can arise from acute biomechanical stresses or chronic overuse and can occur across different age groups. Common injuries include sprains, fractures, and muscular strains, often resulting from...
2.0K
01:19Naming Skeletal Muscles
4.2K
The naming of the approximately 700 muscles in the human body is based on a set of criteria designed to provide descriptive information about each muscle, making it easier to identify and remember them.
The key factors used in naming muscles include:
The key factors used in naming muscles include:
4.2K