Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Chromogenic substrates for activated partial thromboplastin time testing: are they worth using?

A Tripodi1

  • 1Centro Emofilia e Trombosi Angelo Bianchi Bonomi, Università degli Studi ed Ospedale Maggiore, Milano.

La Ricerca in Clinica E in Laboratorio
|October 1, 1989
PubMed
Summary

Chromogenic substrates offer an alternative for activated partial thromboplastin time (APTT) testing in hemostasis. While effective for many deficiencies, chromogenic APTT shows lower sensitivity to heparin and lupus anticoagulants compared to clotting methods.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Preprocedural prophylaxis with blood products in patients with cirrhosis: Results from a survey of the Italian Association for the Study of the Liver (AISF).

Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·2022
Same author

Implementing pre-procedural thrombopoietin receptor agonists in cirrhotic patients with severe thrombocytopenia: Indiscriminate, selective or unneeded?

Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·2021
Same author

Thrombopoietin receptor agonists before elective invasive procedures in cirrhotic patients with thrombocytopenia: ready to start?

Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·2021
Same author

Evaluation of procoagulant imbalance in Cushing's syndrome after short- and long-term remission of disease.

Journal of endocrinological investigation·2021
Same author

Low drug levels and thrombotic complications in high-risk atrial fibrillation patients treated with direct oral anticoagulants.

Journal of thrombosis and haemostasis : JTH·2018
Same author

Interlaboratory variability in the measurement of direct oral anticoagulants: results from the external quality assessment scheme.

Journal of thrombosis and haemostasis : JTH·2018

Area of Science:

  • Hematology
  • Clinical Chemistry
  • Biochemistry

Background:

  • Chromogenic substrates offer an alternative to traditional clot-based methods in hemostasis testing.
  • This technology is increasingly adopted for specific assays but less so for global screening tests.

Purpose of the Study:

  • To evaluate the clinical validity and application of two chromogenic substrate reagents for activated partial thromboplastin time (APTT) testing.
  • To compare the performance of chromogenic APTT with the conventional clotting method.

Main Methods:

  • Comparative study of two chromogenic substrate reagents against a conventional clotting method for APTT testing.
  • Analysis of plasma samples from patients with congenital deficiencies, on heparin treatment, and with lupus anticoagulant.

Related Experiment Videos

Main Results:

  • Chromogenic APTT detected congenital deficiencies of the intrinsic coagulation pathway similarly to clotting APTT, excluding hypo- and dysfibrinogenemia.
  • Chromogenic methods exhibited reduced responsiveness to heparinization compared to clotting methods.
  • Lupus anticoagulant was detected in most samples from lupus subjects using chromogenic methods, but with lower sensitivity than the clotting method.

Conclusions:

  • Chromogenic APTT is generally suitable for hemostasis testing, providing comparable information to conventional clotting methods.
  • Chromogenic APTT requires improved sensitivity for accurate heparin monitoring and lupus anticoagulant detection.