Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Metabolizable Ultrathin Bi2 Se3 Nanosheets in Imaging-Guided Photothermal Therapy.

Small (Weinheim an der Bergstrasse, Germany)·2016
Same author

Evaluation of Volatile Organic Compounds and Carbonyl Compounds Present in the Cabins of Newly Produced, Medium- and Large-Size Coaches in China.

International journal of environmental research and public health·2016
Same author

[Comparison and optimization of total ionic strength adjustment buffer during detecting fluoride in trace serum sample by fluoride ion selective electrode method].

Wei sheng yan jiu = Journal of hygiene research·2016
Same author

Flexible Transparent Electronic Gas Sensors.

Small (Weinheim an der Bergstrasse, Germany)·2016
Same author

Magnetoresistance in Co/2D MoS2/Co and Ni/2D MoS2/Ni junctions.

Physical chemistry chemical physics : PCCP·2016
Same author

Development and Validation of an Interactive Efficient Dose Rates Distribution Calculation Program Arshield for Visualization of Radiation Field in Nuclear Power Plants.

Radiation protection dosimetry·2016

Related Experiment Video

Updated: Apr 24, 2026

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells
14:02

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

Published on: April 9, 2018

7.9K

Interfacial Bioorthogonal Cross-Linking.

Han Zhang1, Kevin T Dicker1, Xian Xu1

  • 1Departments of Chemistry and Biochemistry and Materials Science and Engineering, University of Delaware , Newark, Delaware 19716, United States.

ACS Macro Letters
|September 2, 2014
PubMed
Summary
This summary is machine-generated.

Bioorthogonal chemistry enables interfacial cross-linking for creating patterned biomaterials. This rapid reaction forms hydrogel microspheres and channels, useful for cell encapsulation and tissue models.

More Related Videos

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies
10:01

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies

Published on: November 28, 2017

20.5K
Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors
16:16

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors

Published on: September 13, 2013

14.6K

Related Experiment Videos

Last Updated: Apr 24, 2026

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells
14:02

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

Published on: April 9, 2018

7.9K
Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies
10:01

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies

Published on: November 28, 2017

20.5K
Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors
16:16

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors

Published on: September 13, 2013

14.6K

Area of Science:

  • Biomaterials Science
  • Bioorthogonal Chemistry
  • Chemical Engineering

Background:

  • Bioorthogonal chemistry facilitates reactions between molecules that do not interfere with native biochemical processes.
  • Interfacial reactions offer precise control over material formation and patterning.
  • Hydrogels are widely used in tissue engineering and drug delivery due to their biocompatibility and tunable properties.

Purpose of the Study:

  • To introduce and demonstrate interfacial bioorthogonal cross-linking for biomaterial fabrication.
  • To utilize the rapid click chemistry between strained *trans*-cyclooctene (TCO) and tetrazine (Tz) derivatives.
  • To create patterned biomaterials, including cell-laden microspheres and hydrogel channels, with high resolution.

Main Methods:

  • Employing a rapid bioorthogonal reaction (k2 = 284000 M−1 s−1) between TCO and Tz functional groups.
  • Utilizing syringe delivery of Tz-functionalized hyaluronic acid (HA-Tz) into a bis-TCO cross-linker bath for microsphere formation.
  • Reversing the order of addition to fabricate water-filled hydrogel channels.

Main Results:

  • Instantaneous formation of microspheres with cross-linked shells via diffusion-controlled interfacial gelation.
  • Successful introduction of tags with 3D resolution without external triggers or templates.
  • High viability (99%) and proliferation of encapsulated prostate cancer cells within the microspheres, forming aggregated clusters.

Conclusions:

  • Interfacial bioorthogonal cross-linking is a versatile method for fabricating complex biomaterials.
  • The developed technique allows for precise patterning and 3D structuring of hydrogels.
  • This approach shows promise for creating cell-instructive matrices for advanced in vitro tissue models.