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Quantitative analysis of γ-oryzanol content in cold pressed rice bran oil by TLC-image analysis method
Apirak Sakunpak1, Jirapornchai Suksaeree1, Chaowalit Monton1
1Faculty of Pharmacy, Rangsit University, Pathum Thani, 12000, Thailand ; Sino-Thai Traditional Medicine Research Center, Herbal Medicinal Products Research and Development Center (Cooperation between Rangsit University and Harbin Institute of Technology and Heilongjiang University of Chinese Medicine), Rangsit University, Pathum Thani, 12000, Thailand.
Objective:
To develop and validate an image analysis method for quantitative analysis of γ-oryzanol in cold pressed rice bran oil.
Methods:
TLC-densitometric and TLC-image analysis methods were developed, validated, and used for quantitative analysis of γ-oryzanol in cold pressed rice bran oil. The results obtained by these two different quantification methods were compared by paired t-test.
Results:
Both assays provided good linearity, accuracy, reproducibility and selectivity for determination of γ-oryzanol.
Conclusions:
The TLC-densitometric and TLC-image analysis methods provided a similar reproducibility, accuracy and selectivity for the quantitative determination of γ-oryzanol in cold pressed rice bran oil. A statistical comparison of the quantitative determinations of γ-oryzanol in samples did not show any statistically significant difference between TLC-densitometric and TLC-image analysis methods. As both methods were found to be equal, they therefore can be used for the determination of γ-oryzanol in cold pressed rice bran oil.
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