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Updated: Apr 24, 2026

Biochemical Reconstitution of Steroid Receptor•Hsp90 Protein Complexes and Reactivation of Ligand Binding
Published on: September 21, 2011
Cofactor profiling of the glucocorticoid receptor from a cellular environment
Sofie J Desmet1, Lien Dejager, Dorien Clarisse
1Cytokine Receptor Lab, VIB Department of Medical Protein Research, Department of Biochemistry, University of Ghent, Albert Baertsoenkaai 3, 9000, Ghent, Belgium.
Abstract:
The Microarray Assay for Realtime Coregulator-Nuclear receptor Interaction (MARCoNI) technology allows the identification of nuclear receptor-coregulator interactions via flow-through microarrays. As such, differences in the coregulator profile between distinct nuclear receptors or of a single receptor in agonist or antagonist mode can be investigated, even in a single run. In this chapter, the method how to perform these peptide microarrays with cell lysates containing the overexpressed glucocorticoid receptor is described, as well as the influence of assay parameters, variations to the protocol, and data analysis.
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