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Expression of a staphylokinase, a thrombolytic agent in Arabidopsis thaliana
Aneta Wiktorek-Smagur1, Katarzyna Hnatuszko-Konka, Aneta Gerszberg
1Department of Genetics and Plant Molecular Biology and Biotechnology, The University of Lodz, S. Banacha 12/16, 90-237, Lodz, Poland, a_wiktorek@poczta.onet.pl.
Abstract:
A gene encoding staphylokinase from Staphylococcus aureus was cloned into the plant transformation binary vector pCAMBIA 1304. The transgene was introduced into the genome of A. thaliana via in planta Agrobacterium tumefaciens-mediated genetic transformation. The presence of the staphylokinase gene was confirmed by PCR in 60% of the investigated plants. The presence of the fusion protein (119 kDa) was confirmed by SDS-PAGE and Western blot analysis in protein extracts from putative transgenics. Furthermore, the amidolytic assay confirmed the activity of SAK in protein extracts in 23 out of 45 transgenic lines of A. thaliana plants.
