Related Experiment Video
Updated: Dec 28, 2025

Author Spotlight: Process Development for the Spray-Drying of Probiotic Bacteria and Evaluation of the Product Quality
Published on: April 7, 2023
[Effect of temperature on prodigiosin synthesis in Serratia marcecens]
Objective:
To study the effect of temperature on prodigiosin synthesis in Serratia marcecens JNB5-1.
Methods:
Different proteins obtained after culturing S. marcecens JNB5-1 at 28 degrees C and 37 degrees C were separated and identified through two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization-time of flight-mass spectrometry. Subsequently, 7 of the proteins were further analyzed by real-time qPCR.
Results:
We identified 16 different proteins. Among them, prodigiosin biosynthetic enzymes (O-methyl transferase and Oxidoreductase) and proteins related with the precursor substances (proine, methionine, serine, 2-Octenal and Malonyl-CoA) of the prodigiosin biosynthetic were significantly down-expressed at 37 degrees C. Heat shock protein (Hsp60) and superoxide dismutase were up-expressed at 37degrees C. The results of real-time qPCR show that the mRNA transcriptional levels of o-methyl transferase, oxidoreductase and transketolase at 37 degrees C were all lower than that at 28 degrees C.
Conclusion:
S. marcecens JNB5-1 could produce prodigiosin at lower temperature (28 degrees C) but not at higher temperature (37 degrees C). Higher temperature probably inhibited the expression of enzymes related with prodigiosin biosynthesis.
More Related Videos
Related Concept Videos
Physical Methods for Controlling Microbial Growth: Temperature
Factors Influencing Microbial Growth: Temperature
Hyperthermophilic Bacteria
Effect of Temperature Change on Reaction Rate
Temperature Dependence on Reaction Rate
Atoms, molecules, or ions must collide before they can react with each other. Atoms must be close together to form chemical bonds. This premise is the basis for a theory that explains many observations regarding chemical kinetics, including factors affecting reaction rates.
The collision theory is based on the postulates that (i) the reaction rate is proportional to the rate of reactant collisions, (ii) the reacting species collide in an orientation allowing contact between...
Effects of Temperature on Free Energy

