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Bimix antimicrobial scaffolds for regenerative endodontics.
Jadesada Palasuk1, Krzysztof Kamocki2, Lauren Hippenmeyer2
1Department of Restorative Dentistry, Division of Dental Biomaterials, Indiana University School of Dentistry (IUSD), Indianapolis, Indiana; Department of Endodontics Indiana University School of Dentistry (IUSD), Indianapolis, Indiana.
New polydioxanone scaffolds loaded with antibiotics show promise for regenerative endodontics. These bimix antibiotic fibers effectively inhibit bacterial growth while maintaining good cytocompatibility, offering a potential new drug delivery system.
Area of Science:
- Biomaterials Science
- Dental Regenerative Medicine
- Antimicrobial Drug Delivery
Background:
- Bacterial elimination is crucial for successful root canal regeneration.
- Polydioxanone-based scaffolds offer a potential delivery system for antibiotics.
- Investigating novel materials for enhanced endodontic treatments is essential.
Purpose of the Study:
- To synthesize and evaluate the antimicrobial effectiveness and cytocompatibility of bimix antibiotic-containing polydioxanone scaffolds.
- To mimic the double antibiotic paste (DAP) using electrospun fibers.
- To assess the potential of these scaffolds in regenerative endodontics.
Main Methods:
- Antibiotic-containing (metronidazole [MET] and ciprofloxacin [CIP]) polymer solutions were electrospun into fibers.
- Fiber morphology, chemical characteristics, and tensile strength were analyzed.
- Antimicrobial efficacy against Enterococcus faecalis, Porphyromonas gingivalis, and Fusobacterium nucleatum was tested in vitro.
- Cytotoxicity was evaluated using human dental pulp stem cells.
Main Results:
- Electrospinning successfully produced nanoscale antibiotic-containing fibers.
- Scaffolds with a 1:1 MET/CIP ratio exhibited significantly higher tensile strength.
- Antibiotic-loaded scaffolds effectively inhibited the growth of all tested bacteria.
- While slight reductions in cell viability were observed, scaffolds were not cytotoxic.
Conclusions:
- Nanofibrous scaffolds incorporating multiple antibiotics show significant potential for regenerative endodontics.
- These scaffolds represent a promising drug delivery system for endodontic applications.
- Further development could lead to improved outcomes in root canal regeneration.
