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Synthesis of Aptamer-PEI-g-PEG Modified Gold Nanoparticles Loaded with Doxorubicin for Targeted Drug Delivery
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Nucleotropic doxorubicin nanoparticles decrease cancer cell viability, destroy mitochondria, induce autophagy and

Anna M Friedhuber1, Vijay Chandolu, Somkamon Manchun

  • 1Department of Pathology, University of Melbourne, Melbourne, Australia.

The Journal of Pharmacy and Pharmacology
|September 12, 2014
PubMed
Abstract

Insights

A novel nanoparticle drug delivery system (DNP) significantly boosts doxorubicin

Area of Science:

  • Nanotechnology
  • Drug Delivery Systems
  • Oncology

Background:

  • Doxorubicin (Dox) is a vital chemotherapy drug for various cancers.
  • Significant side effects limit Doxorubicin's clinical use.
  • Developing advanced drug delivery systems (DDSs) for Doxorubicin is crucial.

Purpose of the Study:

  • To develop and evaluate a novel nucleotropic Doxorubicin-loaded nanoparticle (DNP) DDS.
  • To assess the DNP's efficacy in enhancing Doxorubicin's anti-cancer activity.
  • To investigate the DNP's mechanism of action and in vivo performance.

Main Methods:

  • Formulation of Doxorubicin-loaded nanoparticles (DNPs) using vortex-assisted complex coacervation.
  • In vitro evaluation of DNP's cell-inhibitory activity against human cancer cell lines.
  • In vivo assessment of DNP's anti-tumor efficacy in a mouse model of osteosarcoma.

Main Results:

  • DNPs demonstrated a 300-fold enhancement in cell-inhibitory activity against multiple cancer cell lines.
  • In vivo studies showed a 10-fold increase in efficacy against osteosarcoma.
  • DNPs localized to the endoplasmic reticulum, disrupted mitochondria, and entered the nucleus, inducing autophagy.

Conclusions:

  • The developed nucleotropic DNP DDS is a promising, cost-effective, and non-biohazardous approach.
  • DNPs significantly enhance Doxorubicin's delivery and anti-cancer activity.
  • This DDS holds potential for improved clinical application in treating various cancers.
Keywords:
cancerdoxorubicinefficacynanoparticlenucleus

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