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Visualizing Yeast Organelles with Fluorescent Protein Markers

Visualizing Yeast Organelles with Fluorescent Protein Markers

Published on: April 20, 2022

Single yeast cell imaging.

Heimo Wolinski¹, Sepp D Kohlwein

¹Institute of Molecular Biosciences, University of Graz, Graz, Austria.

Methods in Molecular Biology (Clifton, N.J.)

|September 13, 2014

Summary

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This study details live yeast cell imaging techniques using fluorescent dyes and GFP-tagged proteins. It addresses common pitfalls and limitations for improved cellular process research and imaging screens.

Area of Science:

Life Sciences
Cell Biology
Microscopy

Background:

Microscopy is crucial for life science research.
Yeast serves as a model organism for studying cellular processes.
Yeast is widely used in imaging-based screens.

Purpose of the Study:

To describe methods for live yeast cell imaging.
To provide guidance on labeling and maintaining yeast cell viability during microscopy.
To identify common pitfalls and artifacts in yeast imaging.

Main Methods:

Labeling live yeast cells with organelle-specific fluorescent dyes.
Utilizing GFP-tagged proteins for cellular component visualization.
Maintaining cell viability during extended microscopy sessions.

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Visualizing Yeast Organelles with Fluorescent Protein Markers

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Published on: January 23, 2012

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Applying bleaching techniques to study dynamic cellular processes.

Main Results:

Established protocols for labeling and imaging live yeast cells.
Identified potential artifacts related to labeling and cell physiology.
Described the application and limitations of bleaching techniques for dynamic studies.

Conclusions:

Provides a comprehensive guide for live yeast cell imaging.
Offers solutions for common challenges in yeast microscopy.
Enhances the utility of yeast as a model for cellular research.