Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Reconstruction of Signal using Interpolation01:10

Reconstruction of Signal using Interpolation

910
Signal processing techniques are essential for accurately converting continuous signals to digital formats and vice versa. When a continuous signal is sampled with a period T, the resulting sampled signal exhibits replicas of the original spectrum in the frequency domain, spaced at intervals equal to the sampling frequency. To handle this sampled signal, a zero-order hold method can be applied, which creates a piecewise constant signal by retaining each sample's value until the next...
910
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

5.7K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
5.7K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.3K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

pH-Responsive Core-Shell Polymer Capsules via Liquid-Liquid Encapsulation with Decoupled Release Onset and Diffusion Kinetics.

ACS applied bio materials·2026
Same author

Creating Tunable Low-Surface-Tension-Liquid Capsules via Impact-Driven Liquid-Liquid Encapsulation.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

Alginate cryogel beads for effectively aggregating nanoplastics for water remediation.

Communications chemistry·2025
Same author

Solvent-Induced Triradial Pattern Formation on Solid-Supported Viscoelastic Thin Films and Gels.

ACS applied materials & interfaces·2025
Same author

Controlled Release of Dyes from Hydrogel Cargoes through pH and Magnetic Stimuli.

Langmuir : the ACS journal of surfaces and colloids·2025
Same author

Dynamics of an Artificial Tear Film on Contact Lenses in Response to a Moving Force Mimicking Fingertip Application.

ACS applied materials & interfaces·2025

Related Experiment Video

Updated: Apr 24, 2026

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
14:09

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

7.8K

Stochastic reconstruction using multiple correlation functions with different-phase-neighbor-based pixel selection.

Lalit M Pant1, Sushanta K Mitra1, Marc Secanell1

  • 1Department of Mechanical Engineering, University of Alberta, Edmonton, Canada, T6G 2G8.

Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics
|September 13, 2014
PubMed
Summary
This summary is machine-generated.

A new reconstruction method using threshold accepting (TA) and different-phase-neighbor (DPN) pixel swapping significantly reduces energy and improves convergence time for two-phase materials. This advanced technique enhances structural accuracy in material reconstructions.

More Related Videos

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment
07:12

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

Published on: January 6, 2026

743
Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy
14:23

Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy

Published on: March 6, 2018

10.5K

Related Experiment Videos

Last Updated: Apr 24, 2026

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
14:09

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

7.8K
Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment
07:12

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

Published on: January 6, 2026

743
Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy
14:23

Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy

Published on: March 6, 2018

10.5K

Area of Science:

  • Materials Science
  • Computational Modeling
  • Image Reconstruction

Background:

  • Accurate reconstruction of heterogeneous two-phase mediums is crucial for understanding material properties.
  • Conventional simulated annealing methods can be computationally intensive and may not always yield optimal structures.

Purpose of the Study:

  • To develop an improved reconstruction methodology for two-dimensional heterogeneous two-phase mediums.
  • To enhance the efficiency and accuracy of structural reconstruction compared to existing methods.

Main Methods:

  • A novel reconstruction approach combining threshold accepting (TA) energy minimization and different-phase-neighbor (DPN) pixel swapping.
  • Development of an in-house solver for implementing the proposed reconstruction algorithm.
  • Investigation of multiple correlation functions (two-point correlation and lineal path functions) for reconstruction accuracy.

Main Results:

  • The proposed TA and DPN method achieved up to an order of magnitude reduction in energy compared to conventional simulated annealing.
  • A 50% improvement in convergence time was observed with the optimized threshold tolerance value.
  • Reconstructions utilizing both two-point correlation and lineal path functions demonstrated the highest accuracy.

Conclusions:

  • The developed TA and DPN methodology offers a more efficient and effective approach for reconstructing two-phase materials.
  • The choice of correlation functions significantly impacts the accuracy of the reconstructed material structure.
  • This improved algorithm provides a robust tool for studying microstructural characteristics and their influence on material behavior.