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Nanochannel-based electrochemical assay for transglutaminase activity
Iñigo Fernández1, Alfredo Sánchez, Paula Díez
1Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, 28040-Madrid, Spain. pingarro@quim.ucm.es rvillalonga@quim.ucm.es.
Abstract:
A novel electrochemical assay to quantify transglutaminase activity is reported. The assay is based on the enzyme-controlled diffusion of Fe(CN)6(3-/4-) through amino-functionalized nanochannels of a mesoporous silica thin film on a Au surface in the presence of N-benzyloxycarbonyl-L-glutaminylglycine.

