Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA-seq03:21

RNA-seq

9.2K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.2K
Rab Cascades01:25

Rab Cascades

2.8K
Rab GTPases act in a regulated cascade during membrane fusion, helping the lipid bilayers mix. The Rab family of proteins are active when bound to GTP, and inactive when bound to GDP. Hence, they act as guanine nucleotide-dependent molecular switches. Rab-GTP recognizes and binds to long or short-range tethering proteins to capture the target vesicle. These tethers coordinate with SNAREs on the vesicle and the target membrane to assemble the trans SNARE complex that locks the mixing bilayers.
2.8K
Bacterial RNA Polymerase00:43

Bacterial RNA Polymerase

19.9K
Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
19.9K
RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

5.9K
Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific...
5.9K
RNA Structure01:23

RNA Structure

68.6K
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
68.6K
RNA Structure01:19

RNA Structure

6.5K
The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
6.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

circVDJ-seq for T cell clonotype detection in single-cell and spatial multi-omics.

Genome medicine·2026
Same author

The history and function of a circular RNA.

Nature communications·2026
Same author

Accelerating Leigh syndrome drug discovery through deep learning screening in brain organoids.

Nature communications·2026
Same author

Pluripotent stem-cell-based screening uncovers sildenafil as a mitochondrial disease therapy.

Cell·2026
Same author

miRNA regulation in brain tissue space: the 3'UTR perspective.

RNA (New York, N.Y.)·2026
Same author

Mass spectrometry-based peripheral blood proteomics for biomarker discovery in idiopathic pulmonary fibrosis.

Respiratory research·2025

Related Experiment Video

Updated: Apr 23, 2026

Identification of Circular RNAs using RNA Sequencing
08:25

Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

11.8K

circBase: a database for circular RNAs.

Petar Glažar1, Panagiotis Papavasileiou1, Nikolaus Rajewsky2

  • 1Max Delbrück Center for Molecular Medicine, 13125 Berlin, Germany.

RNA (New York, N.Y.)
|September 20, 2014
PubMed
Summary

Researchers have identified thousands of circular RNAs (circRNAs) in animals. A new database, circBase, offers access to circRNA data and tools for identifying these molecules.

Keywords:
circular RNAdatabasegene expression

More Related Videos

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions
10:27

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

Published on: October 21, 2022

1.9K
Genome-wide Purification of Extrachromosomal Circular DNA from Eukaryotic Cells
14:26

Genome-wide Purification of Extrachromosomal Circular DNA from Eukaryotic Cells

Published on: April 4, 2016

28.3K

Related Experiment Videos

Last Updated: Apr 23, 2026

Identification of Circular RNAs using RNA Sequencing
08:25

Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

11.8K
In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions
10:27

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

Published on: October 21, 2022

1.9K
Genome-wide Purification of Extrachromosomal Circular DNA from Eukaryotic Cells
14:26

Genome-wide Purification of Extrachromosomal Circular DNA from Eukaryotic Cells

Published on: April 4, 2016

28.3K

Area of Science:

  • Molecular Biology
  • Bioinformatics

Background:

  • Thousands of circular RNAs (circRNAs) have been identified in animal species.
  • circRNAs exhibit high stability and specific expression patterns.
  • The functions of circRNAs remain largely unknown, but their characteristics suggest potential as biomarkers.

Purpose of the Study:

  • To create a centralized, unified database of known circRNAs.
  • To provide tools for identifying known and novel circRNAs from sequencing data.
  • To facilitate access and browsing of circRNA data within a genomic context.

Main Methods:

  • Development of the circBase database and website.
  • Integration and unification of circRNA datasets from multiple laboratories.
  • Inclusion of scripts for circRNA identification in sequencing data.

Main Results:

  • circBase provides a comprehensive, browsable, and downloadable repository of circRNA data.
  • The database includes evidence supporting the expression of identified circRNAs.
  • Accessible scripts enable researchers to identify circRNAs in their own sequencing experiments.

Conclusions:

  • circBase serves as a valuable resource for researchers studying circRNAs.
  • The database supports further investigation into the roles and potential applications of circRNAs.
  • Availability of unified data and identification tools promotes circRNA research.