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Updated: Apr 23, 2026

Radioactive in situ Hybridization for Detecting Diverse Gene Expression Patterns in Tissue
Published on: April 27, 2012
RNA fluorescence in situ hybridization in cultured mammalian cells
Vidisha Tripathi1, Jingyi Fei, Taekjip Ha
1Department of Cell and Developmental Biology, University of Illinois at Urbana-Champaign, 601 S. Goodwin Avenue, Urbana, IL, 61801, USA.
Abstract:
It is now clear that long noncoding RNAs (lncRNAs) regulate a number of aspects of nuclear organization and gene expression. An important tool for the study of the distribution and function of lncRNAs is RNA fluorescence in situ hybridization (RNA-FISH). The protocols presented in this chapter describe this method in detail and also mention a number of critical points that must be considered when performing this technique.
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