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Laminar Flow-based Assays to Investigate Leukocyte Recruitment on Cultured Vascular Cells and Adherent Platelets
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Methods for assessing the effects of galectins on leukocyte trafficking
Beatrice R Gittens1, Rachael D Wright, Dianne Cooper
1William Harvey Research Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, Charterhouse Square, EC1M 6BQ, London, UK.
Methods in Molecular Biology (Clifton, N.J.)
|September 26, 2014
Abstract
None:
Numerous protocols exist for investigating leukocyte recruitment both in vitro and in vivo. Here we describe three of these methods; an in vitro flow chamber assay, intravital microscopy, and zymosan-induced peritonitis, and give details as to how they can be used to study the actions of galectins on this crucial process.
