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Complete nucleotide sequence of wound tumor virus genomic segments encoding nonstructural polypeptides
1Department of Cell and Developmental Biology, Roche Institute of Molecular Biology, Roche Research Center, Nutley, New Jersey 07110.
Virology
|July 1, 1989
Summary
Sequence analysis of wound tumor virus (WTV) reveals similarities with rice dwarf virus, suggesting a common origin. This research aids in understanding phytoreovirus functions through sequence databases.
Area of Science:
- Virology
- Molecular Biology
- Genomics
Background:
- Wound tumor virus (WTV) is a significant plant pathogen.
- Understanding WTV's genomic structure is crucial for disease management.
Purpose of the Study:
- To complete the genomic sequence analysis of WTV nonstructural polypeptides.
- To compare WTV sequences with other Phytoreovirus members to infer evolutionary relationships.
Main Methods:
- Completed nucleotide sequence determination for WTV segments S4, S6, S9, and S10.
- Compared WTV segment S10 sequence with rice dwarf virus segment S10.
Main Results:
- The complete nucleotide sequences for WTV segments S4, S6, S9, and S10 were determined.
- Significant nucleotide (54.9%) and amino acid (30.6%) similarity was found between WTV and rice dwarf virus S10 segments.
- Despite differences in host and vector range, sequence similarity suggests a common viral origin.
Conclusions:
- The genomic sequence data provides a foundation for understanding WTV gene functions.
- Sequence similarity between WTV and rice dwarf virus supports their shared evolutionary history.
- A phytoreovirus sequence database can aid in predicting viral gene product functions.