Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

20S small nuclear ribonucleoprotein U5 shows a surprisingly complex protein composition.

M Bach1, G Winkelmann, R Lührmann

  • 1Max-Planck-Institut für Molekulare Genetik, Otto-Warburg-Laboratorium, Berlin, Federal Republic of Germany.

Proceedings of the National Academy of Sciences of the United States of America
|August 1, 1989
PubMed
Summary

U5 small nuclear ribonucleoprotein (snRNP) contains unique proteins, suggesting they are crucial for its splicing function. These U5-specific proteins, identified via immunoaffinity and cofractionation, are structurally distinct and essential for the particle

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

ISCEV standard for clinical visual evoked potentials (2025 update).

Documenta ophthalmologica. Advances in ophthalmology·2025
Same author

Morphometric analysis of the abducens nerve in the petroclival region.

Frontiers in surgery·2025
Same author

New-generation 0.55T MRI in patients with total hip arthroplasty: a comparison with 1.5T MRI.

Clinical radiology·2024
Same author

Fracture risk prediction in postmenopausal women from GO Study: the comparison between FRAX, Garvan, and POL-RISK algorithms.

Archives of osteoporosis·2024
Same author

ISCEV standard for clinical pattern electroretinography (2024 update).

Documenta ophthalmologica. Advances in ophthalmology·2024
Same author

Optimal fracture prediction thresholds for therapy onset, established from FRAX and Garvan algorithms: a longitudinal observation of the population representative female cohort from the RAC-OST-POL Study.

Archives of osteoporosis·2023

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Genetics

Background:

  • Small nuclear ribonucleoproteins (snRNPs) are essential for pre-mRNA splicing.
  • U5 snRNP is one of the major snRNPs involved in the splicing process.
  • The protein composition of U5 snRNP and its specific components remain incompletely understood.

Purpose of the Study:

  • To characterize the unique protein components of U5 small nuclear ribonucleoprotein (snRNP).
  • To investigate the specificity and structural integrity of U5-specific proteins.
  • To elucidate the potential role of U5-specific proteins in the splicing mechanism.

Main Methods:

  • Purification of U5 snRNP from HeLa nuclear extracts.
  • Immunoaffinity chromatography using anti-2,2,7-trimethylguanosine antibodies.

Related Experiment Videos

  • Glycerol gradient centrifugation and chromatography for protein fractionation.
  • Western blotting with autoimmune sera to identify specific protein targets.

    • Main Results:

    • U5 snRNP contains common snRNP proteins (B', B, D, D', E, F, G) and unique proteins (40, 52, 100, 102, 116, 200 kDa).
    • U5-specific proteins were selectively eluted and cofractionated with U5 snRNP particles (20S).
    • U5 snRNPs lacking these proteins exhibited lower sedimentation values (8-10S).
    • Autoimmune sera identified the 100- and 200-kDa proteins as U5-specific, confirming their structural distinctness.

    Conclusions:

    • U5 snRNP possesses a distinct set of unique proteins, including high molecular mass components.
    • These U5-specific proteins are integral to the U5 snRNP particle and are structurally independent.
    • The proteinaceous nature of U5 snRNP suggests a primary role in splicing mediated by its unique protein constituents.