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Updated: Apr 23, 2026

Bottom-up and Shotgun Proteomics to Identify a Comprehensive Cochlear Proteome
Published on: March 7, 2014
[Proteomic methods of protein separation and analysis]
Abstract:
The review briefly presents principles and stages of execution of such highly effective methods of protein separation as capillary, 2D gel-electrophoresis, liquid chromatography, the possibility of their successful combination with tandem mass-spectrometry and application in various fields of proteomics. The main problems of proteomic analysis as well as ways of solving by using mass-spectrometry are examined. 2 main approaches during protein identification are described in the review, characteristics and possibilities of various top-down and bottom-up proteomic analytical programs are provided.
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