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Tissue Engineering by Intrinsic Vascularization in an In Vivo Tissue Engineering Chamber
Published on: May 30, 2016
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In vivo remodelling of vascularizing engineered tissues
M Dean Chamberlain1, Michael E D West, Gabrielle C Lam
1Institute of Biomaterials and Biomedical Engineering, University of Toronto, 164 College St., Toronto, ON, M5S 3G9, Canada.
Annals of Biomedical Engineering
|October 10, 2014
Summary
Host response drives beneficial tissue remodelling for vascularized tissue creation. Understanding the complex interactions within engineered tissue constructs is key to harnessing this process for regenerative medicine.
Area of Science:
- Tissue Engineering and Regenerative Medicine
- Biomaterials Science
- Immunology and Inflammation
Background:
- Vascularized tissue creation relies on in vivo host response and tissue remodeling.
- The host response is a complex interaction between biomaterials, cellular components, and host factors.
- This process shares similarities with wound healing and tumor development.
Purpose of the Study:
- To elucidate the characteristics and dynamics of host-driven tissue remodeling.
- To understand how biomaterial and cellular components modulate this response.
- To highlight the roles of hypoxia, macrophage recruitment, and paracrine signaling.
Main Methods:
- Utilized a bottom-up approach with modular tissue constructs.
- Constructs comprised mesenchymal stromal cells (MSC) within endothelial cell (EC)-covered collagen gel rods.
- Investigated the influence of hypoxia, macrophage recruitment, and paracrine signaling.
Main Results:
- Demonstrated that a beneficial tissue remodeling response leads to vascularized tissue formation.
- Highlighted the intricate interplay between construct components and host environment.
- Identified key cellular and molecular mechanisms governing the host response.
Conclusions:
- The host response is critical for successful in vivo vascularization and tissue integration.
- Modular constructs provide a platform to study and engineer this complex remodeling process.
- Further research into hypoxia, macrophages, and paracrine signaling can optimize tissue engineering strategies.
