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Updated: Apr 22, 2026

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets

Published on: October 3, 2014

Cell sheet-based tissue engineering for fabricating 3-dimensional heart tissues.

Tatsuya Shimizu¹

¹Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University.

Circulation Journal : Official Journal of the Japanese Circulation Society

|October 17, 2014

Summary

This summary is machine-generated.

Cell sheet tissue engineering advances regenerative medicine by creating vascularized 3D cardiac tissues. This technique enables thicker, functional heart tissue fabrication for treating heart disease.

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Fabrication of 3D Cardiac Microtissue Arrays using Human iPSC-Derived Cardiomyocytes, Cardiac Fibroblasts, and Endothelial Cells

Fabrication of 3D Cardiac Microtissue Arrays using Human iPSC-Derived Cardiomyocytes, Cardiac Fibroblasts, and Endothelial Cells

Published on: March 14, 2021

Area of Science:

Regenerative Medicine
Tissue Engineering
Biotechnology

Background:

Tissue engineering is crucial for regenerative medicine, offering advantages over cell injection for tissue repair.
Scaffold-based methods are common, but cell sheet-based techniques offer direct transplantation and clinical application.
Cardiac cell sheets can form pulsatile tissue, but vascularization limits thickness; coculture with endothelial cells is key.

Purpose of the Study:

To develop methods for fabricating thick, vascularized 3D cardiac tissues using cell sheet engineering.
To investigate the formation of functional blood vessels within engineered cardiac constructs.
To advance cell sheet-based tissue engineering for potential clinical applications in treating heart disease.

Main Methods:

Harvesting cell sheets from temperature-responsive culture dishes.
Coculturing cardiac cells with endothelial cells within triple-layer cell sheets.
Layering cell sheets onto vascular beds and perfusing them in novel bioreactor systems.

Main Results:

Successfully formed thick, vascularized cardiac tissue in vitro and in vivo.
Demonstrated endothelial cell migration into vascular beds to form perfusable blood vessels.
Achieved fabrication of 3D cardiac tissues exceeding the limitations of simple cell sheet stacking.

Conclusions:

Cell sheet-based tissue engineering holds significant potential for creating complex 3D cardiac tissues.
This approach can overcome limitations in vascularization, enabling thicker tissue constructs.
The technology may contribute to future treatments for severe heart diseases and the development of human tissue models.