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Related Concept Videos

In-situ Hybridization02:31

In-situ Hybridization

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In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
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FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

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Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
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Hybrid Zones02:29

Hybrid Zones

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Hybrid zones are narrow regions where two closely related species interact, mate, and produce hybrids. Relative to either parent species, hybrids may possess distinct phenotypic or genetic differences that impact their survival and reproductive success. The genetic variances introduced by hybridization influence species diversity and speciation processes within the hybrid zone.
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Hybridoma Technology01:31

Hybridoma Technology

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Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation,...
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Southern Blot02:57

Southern Blot

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Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...
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Trihybrid Crosses02:27

Trihybrid Crosses

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Trihybrid Crosses
Some of Mendel’s crosses examined three pairs of contrasting characteristics. Such a cross is called a trihybrid cross. A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs. green).
The F1 generation plants of a trihybrid cross are heterozygous for all three traits and produce eight gametes. Upon self-fertilization, these gametes have an equal...
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Related Experiment Video

Updated: Apr 21, 2026

Novel and Innovative Hybrid Technique for Type A Aortic Dissection
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Hybrid procedure.

Jagdish Hiremath, Kaushik Sheth

    The Journal of the Association of Physicians of India
    |October 21, 2014
    PubMed
    Summary

    Hybrid coronary revascularisation offers a less invasive option for treating complex multivessel coronary artery disease (CAD) in select patients. This approach combines surgical and percutaneous methods for improved outcomes.

    Area of Science:

    • Cardiovascular Medicine
    • Interventional Cardiology
    • Cardiac Surgery

    Background:

    • The aging population and rising prevalence of coronary risk factors like diabetes mellitus contribute to an increase in multivessel coronary artery disease (CAD).
    • Traditional treatments for multivessel CAD include percutaneous coronary intervention (PCI) and coronary artery bypass graft surgery (CABG).
    • Challenges in PCI or CABG suitability and a preference for minimally invasive procedures necessitate alternative treatment strategies.

    Observation:

    • Hybrid coronary revascularisation (HCR) is emerging as a viable option for specific multivessel CAD patients.
    • This technique may be particularly beneficial for individuals with technical limitations for standard PCI or CABG.
    • HCR aims to minimize invasiveness while effectively revascularizing the coronary arteries.

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    Findings:

    • The case study details patient selection criteria for HCR.
    • It outlines the procedural aspects of this hybrid approach for treating multivessel CAD.
    • Successful application of HCR demonstrates its potential in complex cases.

    Implications:

    • HCR represents a significant advancement in the treatment of multivessel coronary artery disease.
    • Careful patient selection is crucial for optimizing outcomes with this hybrid strategy.
    • Further research and clinical experience will define the broader role of HCR in cardiovascular care.