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Updated: Apr 21, 2026
Noninvasive Sampling of Mucosal Lining Fluid for the Quantification of In Vivo Upper Airway Immune-mediator Levels
Published on: August 7, 2017
[Quantitative analysis of interleukin-5 mRNA and protein in nasal polyps]
Objective:
To study the expression of intedeukin-5 (IL-5) mRNA and protein in Nasal polyps and in the inferior turbinate, and to explore the relationship between the expression and the Clinical features.
Method:
Real time fluorescent quantitative PCR(RT-qPCR) and immunohistochemical staining were used to detect the expression of intedeukin-5 (IL-5) mRNA and protein in nasal polyps of 24 cases and in inferior turbinate of 15 cases.
Result:
The expression of IL-5 mRNA in Nasal polyps was 7.52 times higher than that in the inferior turbinate (P < 0.01). The expression rate of IL-5 protein in Nasal polyps was 79.17%, significantly higher than that in the inferior turbinate (26.67%) (chi2 = 10.52, P < 0.01). The differece of expression of IL-5 mRNA was not associated with the sexual distinction, unilateral or bilateral nasal polyps and primary or recurrent nasal polyps (P > 0.01), but was associated with the single or multiple nasal polyps, nasal polyps with or without allergic rhinitis and/or asthma. The differece of expression of IL-5 protein was not associated with the sexual distinction, unilateral or bilateral nasal polyps, primary or recurrent nasal polyps, nasal polyps with or without allergic rhinitis and/or asthma, but was associated with the single and multiple nasal polyps.
Conclusion:
The high expression of IL-5 mRNA and protein is closely related with the formation edema and development of nasal polyps. Some clinical features of Nasal polyps related to the expression level of IL-5 in nasal polyps.
Insights
High levels of interleukin-5 (IL-5) mRNA and protein are found in nasal polyps, correlating with polyp formation and clinical features like allergic rhinitis.
Area of Science:
- Immunology
- Otorhinolaryngology
Context:
- Nasal polyps are inflammatory growths in the nasal cavity.
- Interleukin-5 (IL-5) is a key cytokine in allergic inflammation.
Purpose:
- To quantify interleukin-5 (IL-5) mRNA and protein expression in nasal polyps versus inferior turbinate tissue.
- To investigate the association between IL-5 expression and clinical characteristics of nasal polyps.
Summary:
- Real-time quantitative PCR and immunohistochemistry revealed significantly higher IL-5 mRNA and protein levels in nasal polyps compared to inferior turbinate tissue.
- IL-5 expression correlated with polyp multiplicity and the presence of allergic rhinitis and/or asthma, but not with sex, laterality, or recurrence.
Impact:
- Elevated IL-5 expression is implicated in the pathogenesis, edema, and development of nasal polyps.
- Understanding IL-5's role can inform targeted therapies for nasal polyposis.
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