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Updated: Apr 21, 2026

Generation of Mice Derived from Induced Pluripotent Stem Cells
Published on: November 29, 2012
Generation of induced pluripotent stem cells
1Department of Medical Genetics, Mayo Clinic, 200 First Street, SW, Rochester, MN, 55905, USA, deyle.david@mayo.edu.
This chapter details generating induced pluripotent stem cells (iPSCs) from somatic cells using transcription factors. It covers methods for culturing, reprogramming, and preserving iPSCs for disease modeling and therapeutic development.
Area of Science:
- Stem Cell Biology
- Reproductive Biology
- Genetics
Background:
- Induced pluripotent stem cells (iPSCs) are derived from somatic cells reprogrammed via ectopic expression of embryonic transcription factors.
- iPSC technology offers a valuable platform for disease modeling and developing novel therapeutic strategies for human disorders.
Purpose of the Study:
- To provide researchers with a comprehensive guide on generating and maintaining induced pluripotent stem cells (iPSCs).
- To detail established protocols for MEF-conditioned medium production, MEF preparation, iPSC generation via viral vectors, and subsequent cell culture and cryopreservation.
Main Methods:
- Production of mouse embryonic fibroblast (MEF)-conditioned medium.
- Preparation of mitotically arrested MEFs.
- Generation of iPSCs utilizing viral vectors for transcription factor delivery.
- Standard cell culture techniques for iPSC maintenance and passaging.
- Cryopreservation methods for long-term storage of iPSCs.
Main Results:
- The described methods have been successfully implemented in numerous research laboratories globally.
- The protocols facilitate the generation and propagation of iPSCs, enabling their use in various research applications.
Conclusions:
- The provided chapter offers practical, reproducible methods for iPSC generation and handling.
- Alternative methods for transcription factor delivery may be necessary for cell types resistant to viral transduction.
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