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Updated: Apr 21, 2026

Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells
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Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells

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Clinical significance of mTOR and eIF4E expression in invasive ductal carcinoma

Aixia Hu, Miaomiao Sun, Dong Yan

    Tumori
    |October 25, 2014
    PubMed
    Abstract

    Insights

    Increased expression of mTOR and eIF4E in invasive ductal carcinoma may indicate a higher risk of breast cancer occurrence and metastasis. This study analyzed protein levels in patient tissues to understand their clinical significance.

    Area of Science:

    • Oncology
    • Molecular Biology
    • Biochemistry

    Background:

    • Mammalian target of rapamycin (mTOR) is a key regulator of cell growth.
    • Eukaryotic translation initiation factor 4E (eIF4E) and 4E binding protein (4EBP) are downstream effectors in the mTOR signaling pathway, controlling mRNA translation.
    • Aberrant mTOR signaling is implicated in various cancers, including breast cancer.

    Purpose of the Study:

    • To investigate the clinical significance of mTOR, eIF4E, and 4EBP expression in invasive ductal carcinoma.
    • To determine the correlation between the expression of these proteins and clinicopathological features, particularly tumor metastasis.

    Main Methods:

    • Immunohistochemical SP method was used to detect the expression of mTOR, eIF4E, and 4EBPs.
    • Expression levels were analyzed in 45 invasive ductal carcinoma tissue specimens and matched normal breast tissues.
    • Statistical analysis was performed to assess correlations between protein expressions and lymph node metastasis.

    Main Results:

    • Expressions of mTOR, eIF4E, and 4EBPs were significantly elevated in invasive ductal carcinoma compared to normal breast tissue (P <0.05).
    • A positive correlation was observed between mTOR expression and the expression of eIF4E and 4EBP in invasive ductal carcinoma (P <0.05).
    • Higher positive rates of mTOR, eIF4E, and 4EBPs were found in patients with lymph node metastasis than in those without (P <0.05).

    Conclusions:

    • Elevated expression of mTOR and eIF4E in invasive ductal carcinoma is associated with an increased risk of breast cancer occurrence.
    • The findings suggest that mTOR and eIF4E may play a role in the metastasis of breast cancer.

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