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Laboratory Techniques Used to Maintain and Differentiate Biotypes of Vibrio cholerae Clinical and Environmental Isolates

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Vibrio cholerae: Measuring Natural Transformation Frequency.

Samit S Watve¹, Eryn E Bernardy, Brian K Hammer

¹School of Biology, Georgia Institute of Technology, Atlanta, Georgia.

Current Protocols in Microbiology

|November 5, 2014

Summary

This study details methods for measuring natural transformation in Vibrio cholerae. Quantifying this DNA uptake process is crucial for understanding bacterial adaptation and the spread of antibiotic resistance genes.

Keywords:

TfoX Vibrio cholerae chitin competence natural transformation

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Area of Science:

Microbiology
Genetics
Molecular Biology

Background:

Bacteria utilize natural competence to acquire extracellular DNA via a specialized apparatus.
Acquired DNA can be used for DNA repair, nutrition, or integrated into the bacterial chromosome through homologous recombination.
Natural transformation is a key mechanism for horizontal gene transfer, including the spread of antibiotic resistance.

Purpose of the Study:

To describe protocols for quantifying natural transformation frequencies in Vibrio cholerae.
To provide a method for studying DNA uptake in a significant human pathogen.

Main Methods:

Development and description of quantitative protocols for natural transformation.
Application of these protocols to the bacterium Vibrio cholerae.

Main Results:

Successful quantification of natural transformation frequencies in Vibrio cholerae.
Establishment of reliable methods for assessing DNA uptake in this species.

Conclusions:

Natural transformation is a viable process in Vibrio cholerae, contributing to genetic diversity.
The developed protocols enable further research into the role of transformation in bacterial evolution and pathogenicity.