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An integrated microspectrometer for localised multiplexing measurements.

Zhixiong Hu1, Andrew Glidle, Charles Ironside

  • 1Division of Biomedical Engineering, School of Engineering, University of Glasgow, Glasgow, UK. huzhixiong@nim.ac.cn huabing.yin@glasgow.ac.uk.

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|November 5, 2014
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This summary is machine-generated.

We developed a compact Arrayed Waveguide Grating (AWG) microspectrometer for multiplexed fluorescence measurements. This integrated system enables on-chip spectroscopic analysis and portable multi-wavelength detection from single locations.

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Area of Science:

  • Photonics and Microsystems Engineering
  • Biomedical Optics
  • Analytical Chemistry

Background:

  • Multiplexed fluorescence measurements are crucial for various analytical applications.
  • Existing microspectrometers often lack integration and portability for localized analysis.
  • On-chip spectroscopic capabilities are needed for miniaturized sensing platforms.

Purpose of the Study:

  • To develop an integrated lensed Arrayed Waveguide Grating (AWG) microspectrometer.
  • To demonstrate simultaneous, localized multiplexed fluorescence measurements.
  • To integrate the microspectrometer with a microfluidic platform for enhanced analysis.

Main Methods:

  • Fabrication of a compact (1 mm x 1 cm) integrated lensed AWG microspectrometer.
  • Simultaneous measurement of multiple fluorescence signals using intensity readouts from a CCD camera.
  • Integration with a microfluidic platform for focused illumination and enhanced signal collection.

Main Results:

  • Achieved on-chip spectroscopic measurements with a small footprint device.
  • Demonstrated simultaneous and successive localized multiplexed fluorescence detection using a novel "bead-AWG" platform.
  • Enhanced signal collection and spatial resolution through integrated lensing and microfluidics.

Conclusions:

  • The developed AWG microspectrometer enables localized multiplexed fluorescence measurements.
  • Integration with microfluidics allows for analysis of small volume samples in flow.
  • The system provides a basis for portable, multi-wavelength fluorescence detection systems.