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Updated: Apr 21, 2026

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Glycopeptide Capture for Cell Surface Proteomics
Published on: May 9, 2014
10.9K
Using in-solution digestion, peptide fractionation, and a Q exactive mass spectrometer to analyze the proteome of
Georg H H Borner1, Andrew B Fielding2
1Department of Proteomics and Signal Transduction, Max Plank Institute of Biochemistry, 82152 Martinsried, Germany;
Cold Spring Harbor Protocols
|November 5, 2014
Abstract:
The characterization of clathrin-coated vesicles (CCVs), including the effects of genetic or biochemical manipulations on their composition, can be studied by mass spectrometry analysis of HeLa cell fractions enriched for CCVs. This protocol describes the preparation of samples by in-solution proteolytic digest and subsequent peptide fractionation, followed by analysis in a Q Exactive mass spectrometer.

