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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
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Sanger Sequencing01:57

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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
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RNA-seq03:21

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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Targeted DNA Methylation Analysis by Next-generation Sequencing
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Skim-based genotyping by sequencing.

Agnieszka A Golicz1, Philipp E Bayer, David Edwards

  • 1School of Agriculture and Food Sciences, University of Queensland, Brisbane, QLD, Australia.

Methods in Molecular Biology (Clifton, N.J.)
|November 7, 2014
PubMed
Summary
This summary is machine-generated.

Genotyping by sequencing (GBS) combines genotyping and next-generation sequencing for genetic analysis. This method aids in marker discovery, quantitative trait locus analysis, and genomic selection across various plant species.

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Area of Science:

  • Genomics
  • Molecular Biology
  • Plant Science

Background:

  • Genotyping by sequencing (GBS) integrates established genotyping techniques with next-generation sequencing.
  • It offers a novel approach to deciphering genetic variations within populations.

Purpose of the Study:

  • To outline the core methodologies and diverse applications of GBS.
  • To highlight GBS's utility in plant biology research.

Main Methods:

  • DNA sequencing of individuals within a mapping population.
  • Mapping sequencing reads to a reference genome.
  • Single Nucleotide Polymorphism (SNP) calling, filtering, genotyping, and imputation.
  • Haplotype identification and subsequent downstream analyses.

Main Results:

  • GBS facilitates marker discovery, haplotype identification, and recombination characterization.
  • It is applicable to quantitative trait locus (QTL) analysis, genome-wide association studies (GWAS), and genomic selection (GS).
  • Successful applications demonstrated in rice, maize, artichoke, and Arabidopsis thaliana.

Conclusions:

  • GBS is a versatile and powerful tool for genetic analysis in plants.
  • Its application promises significant advancements in understanding plant biology.
  • GBS is expected to yield novel insights into plant genetics and breeding.