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Updated: Apr 21, 2026

Analyzing the Functions of Mast Cells In Vivo Using 'Mast Cell Knock-in' Mice
Published on: May 27, 2015
Regulation of mast cell survival and apoptosis
Christine Möller Westerberg1, Maria Ekoff, Gunnar Nilsson
1Clinical Immunology and Allergy Unit, Department of Medicine, Karolinska University Hospital Solna L2:04, Stockholm, 171 76, Sweden.
Abstract:
One key characteristic of certain mast cell populations is their longevity. Mast cell survival can also be promoted by Fc-receptor activation. Regulation of cell survival and apoptosis is regulated by the Bcl-2 family that consists of pro- and anti-apoptotic proteins. Depending on their relative cellular expression levels, the cells are either rescued or destined for apoptosis. To determine the regulation of mast cell survival and apoptosis, the expression of different Bcl-2 protein family members can be measured by western blot. The amount of viable versus apoptotic cells is decided by AnnexinV/propidium iodide staining, and cell lysates are prepared for western blot analysis from the appropriated time points.
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