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Cryopreservation of human pluripotent stem cells: a general protocol
Takamichi Miyazaki1, Hirofumi Suemori
1Department of Embryonic Stem Cell Research, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawaharacho, Shogoin, Sakyo-Ku, Kyoto, 606-8507, Japan.
Methods in Molecular Biology (Clifton, N.J.)
|November 13, 2014
Summary
Cryopreservation preserves stem cell potential using two methods: rapid vitrification or slow-cooling. Both standardized protocols ensure high survival rates for human pluripotent stem cells (hPSCs).
Area of Science:
- Stem cell biology
- Cryobiology
- Regenerative medicine
Background:
- Stem cells are crucial for tissue repair and disease modeling.
- Maintaining stem cell viability long-term is essential for research and clinical applications.
- Cryopreservation offers a method for semipermanent storage of stem cells.
Purpose of the Study:
- To review and summarize established cryopreservation techniques for human pluripotent stem cells (hPSCs).
- To highlight the effectiveness of current protocols in maintaining hPSC viability.
Main Methods:
- Vitrification: A rapid freeze-thaw process for hPSCs.
- Slow-cooling: A conventional method followed by rapid thawing.
- Standardization and optimization of both protocols.
Main Results:
- Both vitrification and slow-cooling methods have been optimized for hPSC preservation.
- These cryopreservation protocols achieve high survivability rates for hPSCs.
- Standardized methods ensure consistent and reliable stem cell preservation.
Conclusions:
- Cryopreservation is a vital technique for sustaining the potential of hPSCs.
- Established vitrification and slow-cooling methods effectively preserve hPSCs.
- High survivability is achievable with optimized cryopreservation protocols for hPSCs.
