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Gold nanoaggregates for probing single-living cell based on surface-enhanced Raman spectroscopy.

Peng Lu1, Jing Wang1, Jinyong Lin1

  • 1Fujian Normal University, Ministry of Education and Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of Optoelectronic Science and Technology for Medicine, Fuzhou 350007, China.

Journal of Biomedical Optics
|November 13, 2014
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Summary

Gold nanoparticles delivered into cells via electroporation enable intracellular surface-enhanced Raman spectroscopy (SERS). This method shows high signal enhancement and lower toxicity than silver nanoparticles, offering potential in cell biology and biomedicine.

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Area of Science:

  • Nanotechnology
  • Cell Biology
  • Spectroscopy

Background:

  • Surface-enhanced Raman spectroscopy (SERS) is a powerful technique for chemical analysis.
  • Intracellular SERS requires efficient delivery of nanoparticles into living cells.
  • Gold nanoparticles offer unique optical properties for SERS applications.

Purpose of the Study:

  • To develop a method for delivering gold nanoparticles into living cells for intracellular SERS.
  • To characterize the localization and aggregation of gold nanoparticles within cells.
  • To evaluate the SERS signal enhancement and cellular toxicity of gold nanoparticles compared to silver nanoparticles.

Main Methods:

  • Transient electroporation was used to deliver gold nanoparticles into living cells.
  • Transmission electron microscopy (TEM) was employed to determine nanoparticle subcellular localization.
  • Intracellular SERS was performed to analyze cellular chemical compositions.
  • Cellular toxicity was assessed and compared between gold and silver nanoparticles.

Main Results:

  • Gold nanoparticles were successfully delivered into cells, primarily localizing in the cytoplasm as large nanoaggregates.
  • Intracellular SERS demonstrated significant signal enhancement of cellular chemical compositions.
  • Gold nanoparticles exhibited lower cellular toxicity compared to silver nanoparticles.

Conclusions:

  • Electroporation provides rapid and efficient intracellular delivery of gold nanoparticles for SERS.
  • Gold nanostructures are suitable for intracellular SERS, offering high signal enhancement and reduced toxicity.
  • This approach holds significant potential for cell biology and biomedical research.