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Updated: Apr 21, 2026

Laparoscopic Oocyte Retrieval and Cryopreservation during Vaginoplasty for Treatment of Mayer-Rokitansky-Kuster-Hauser Syndrome
Published on: May 10, 2022
New laparoscopic peritoneal pull-through vaginoplasty technique
Pravin Mhatre1, Jyoti Mhatre2, Rakhi Sahu3
1Department of Obstetrics and Gynecology, Seth G.S. Medical College, N. Wadia Hospital, Mumbai, Maharashtra, India ; Consultant Obstetrician and Gynecologist, Kedar Hospital, Mumbai, Maharashtra, India ; Ovarian Transplant Surgeon, Hiranandani Hospital, Mumbai, Maharashtra, India.
Background:
Many reconstructive surgical procedures have been described for vaginal agenesis. Almost all of them are surgically challenging, multi-staged, time consuming or leave permanent scars on abdomen or skin retrieval sites.
Aim:
A new simple technique using laparoscopic peritoneal pull-through in creation of neo vagina has been described.
Material And Methods:
Total of thirty six patients with congenital absence of vagina (MRKH syndrome) were treated with laparoscopic peritoneal pull through technique of Dr. Mhatre between 2003 till 2012. The author has described 3 different techniques of peritoneal vaginoplasty.
Results:
This technique has given excellent results over a period of one to seven years of follow-up. The peritoneal lining changes to stratified squamous epithelium resembling normal vagina and having acidic Ph.
Conclusion:
Apart from giving excellent normal vaginal function, as the ovary became accessible per vaginum three patients underwent ovum retrieval and pregnancy using surrogate mother, thus making this a fertility enhancing procedure.

