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Updated: Apr 20, 2026

Production of Apolipoprotein C-III Knockout Rabbits using Zinc Finger Nucleases
Published on: November 18, 2013
Generation of Induced Pluripotent Stem Cells in Rabbits
Marielle Afanassieff1,2,3, Yann Tapponnier4,5,6, Pierre Savatier4,5,6
1INSERM U846, Bron, 69500, France. marielle.afanassieff@inserm.fr.
Abstract:
We describe a procedure for generating induced pluripotent stem cell lines in rabbits, using retroviral vectors expressing Oct4, Sox2, Klf4, and c-Myc of human origin to reprogram rabbit fibroblasts prepared from an ear skin biopsy. We also provide detailed procedures for characterizing the resulting iPSC lines, including the analysis of pluripotency marker expression by RT-qPCR, immunolabeling, and fluorescent-associated cell sorting, the evaluation of pluripotency by teratoma production and genetic stability by karyotyping.
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