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Related Experiment Video

Updated: Apr 20, 2026

Establishing Organoids from Human Tooth as a Powerful Tool Toward Mechanistic Research and Regenerative Therapy

Establishing Organoids from Human Tooth as a Powerful Tool Toward Mechanistic Research and Regenerative Therapy

Published on: April 13, 2022

Bioengineered post-natal recombinant tooth bud models.

W Zhang¹, B Vázquez¹, P C Yelick¹

¹Department of Oral and Maxillofacial Pathology, Tufts University, Boston, MA, USA.

Journal of Tissue Engineering and Regenerative Medicine

|November 27, 2014

Summary

This summary is machine-generated.

Researchers engineered biological teeth using 3D tissue engineering with porcine and human dental tissues. The study successfully regenerated mineralized dental tissues and tooth crowns, advancing human tooth regeneration models.

Keywords:

adult stem cells bioengineering tissue recombinants tooth bud

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Last Updated: Apr 20, 2026

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Area of Science:

Regenerative Medicine
Biomaterials Science
Developmental Biology

Background:

Current tooth regeneration strategies face challenges in achieving full functionality and size.
Bioengineering approaches offer potential for creating functional biological teeth.
Utilizing postnatal dental tissues simplifies the source material compared to embryonic tissues.

Purpose of the Study:

To investigate the odontogenic potential of intact postnatal porcine dental tissues and human dental pulp cells.
To develop and characterize three-dimensional (3D) tissue-engineered tooth constructs.
To establish reliable models for bioengineered tooth development and regeneration.

Main Methods:

Harvesting intact dental tissues and cell suspensions from postnatal porcine teeth and human third molar wisdom teeth.
Constructing three-dimensional (3D) recombinant tooth constructs using tissue engineering methods.
Culturing constructs in osteogenic media in vitro followed by subcutaneous transplantation in athymic nude rats.
Analyzing the regenerated tissues using X-ray, histological, and immunohistochemical techniques.

Main Results:

The majority of the recombinant tooth structures successfully formed calcified tissues, including osteodentin, dentin cementum, and enamel.
Morphologically typical tooth crowns composed of dentin and enamel were observed in the regenerated structures.
The study demonstrated the formation of mineralized dental tissues from easily obtainable postnatal dental tissues.

Conclusions:

The successful regeneration of mineralized dental tissues and tooth crown structures represents a significant advancement in tooth regeneration research.
The developed 3D tissue engineering models using postnatal tissues are promising for future human tooth regeneration strategies.
This study provides a foundation for establishing robust and reliable models for bioengineered tooth development.