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High-density self-contained microfluidic KOALA kits for use by everyone.

David J Guckenberger1, Erwin Berthier2, David J Beebe3

  • 1Department of Biomedical Engineering, Wisconsin Institutes for Medical Research, University of Wisconsin-Madison, Madison, WI, USA.

Journal of Laboratory Automation
|November 27, 2014
PubMed
Summary

Kit-on-a-lid assay (KOALA) technology offers low-cost, high-density microfluidic platforms for accessible cell-based assays. This method simplifies high-throughput screening and complex assays, reducing infrastructure and training needs.

Keywords:
assaycell basedhigh throughputlow costmicrofluidic

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Area of Science:

  • Biotechnology
  • Cell Biology
  • Assay Development

Background:

  • Cell-based assays are crucial in research but often involve high costs and specialized infrastructure.
  • Maintaining cell cultures and skilled personnel can be prohibitive for some laboratories.

Purpose of the Study:

  • To demonstrate and characterize high-density kit-on-a-lid assay (KOALA) technology for high-throughput applications.
  • To showcase KOALA's potential for accessible, low-cost microfluidic platforms requiring minimal training and infrastructure.

Main Methods:

  • Development and characterization of high-density KOALA microfluidic platforms.
  • Evaluation of assay density comparable to 384-well plates.
  • Assessment of manual usability without liquid-handling equipment.

Main Results:

  • Achieved high assay density on KOALA platforms, rivaling 384-well plates.
  • Demonstrated ease of use with minimal training and no specialized equipment.
  • Showcased potential for complex, multi-step quantitative immunochemistry assays.

Conclusions:

  • KOALA technology provides an accessible, cost-effective solution for high-throughput cell-based assays.
  • This microfluidic platform technology can be readily adopted by diverse research labs, including those with limited resources.
  • KOALA facilitates high-content screening and complex assay execution, expanding research capabilities.