Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Constant Volume Calorimetry02:41

Constant Volume Calorimetry

31.9K
Calorimeters are useful to determine the heat released or absorbed by a chemical reaction. Coffee cup calorimeters are designed to operate at constant (atmospheric) pressure and are convenient to measure heat flow (or enthalpy change) accompanying processes that occur in solution at constant pressure. A different type of calorimeter that operates at constant volume, colloquially known as a bomb calorimeter, is used to measure the energy produced by reactions that yield large amounts of heat and...
31.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hygrothermal Properties and Performance of Bio-Based Insulation Materials Locally Sourced in Sweden.

Materials (Basel, Switzerland)·2024
Same author

Special Issue "Advances in Monitoring Metabolic Activities of Microorganisms by Calorimetry".

Microorganisms·2023
Same author

The effect of reversible permeabilization and post-electroporation resting on the survival of Thai basil (O. Basilicum cv. thyrsiflora) leaves during drying.

Bioelectrochemistry (Amsterdam, Netherlands)·2021
Same author

Inhibition mechanisms of hemoglobin, immunoglobulin G, and whole blood in digital and real-time PCR.

Analytical and bioanalytical chemistry·2018
Same author

Investigation of the metabolic consequences of impregnating spinach leaves with trehalose and applying a pulsed electric field.

Bioelectrochemistry (Amsterdam, Netherlands)·2016
Same author

Calorespirometry of terrestrial organisms and ecosystems.

Methods (San Diego, Calif.)·2014

Related Experiment Video

Updated: Apr 20, 2026

Calorespirometry: A Powerful, Noninvasive Approach to Investigate Cellular Energy Metabolism
09:08

Calorespirometry: A Powerful, Noninvasive Approach to Investigate Cellular Energy Metabolism

Published on: May 31, 2018

8.1K

A method for time-resolved calorespirometry of terrestrial samples.

Lars Wadsö1

  • 1Building Materials, Lund University, Box 118, 221 00 Lund, Sweden.

Methods (San Diego, Calif.)
|December 3, 2014
PubMed
Summary
This summary is machine-generated.

A novel calorespirometry vessel allows simultaneous measurement of heat production, oxygen consumption, and carbon dioxide production in small terrestrial biological samples. This technique provides insights into metabolic activity changes over time.

Keywords:
CalorespirometryIsothermal calorimetryMethodRespirometry

More Related Videos

High-throughput Screening of Recalcitrance Variations in Lignocellulosic Biomass: Total Lignin, Lignin Monomers, and Enzymatic Sugar Release
11:31

High-throughput Screening of Recalcitrance Variations in Lignocellulosic Biomass: Total Lignin, Lignin Monomers, and Enzymatic Sugar Release

Published on: September 15, 2015

10.6K
Measuring Carbon-based Contaminant Mineralization Using Combined CO2 Flux and Radiocarbon Analyses
11:19

Measuring Carbon-based Contaminant Mineralization Using Combined CO2 Flux and Radiocarbon Analyses

Published on: October 21, 2016

12.5K

Related Experiment Videos

Last Updated: Apr 20, 2026

Calorespirometry: A Powerful, Noninvasive Approach to Investigate Cellular Energy Metabolism
09:08

Calorespirometry: A Powerful, Noninvasive Approach to Investigate Cellular Energy Metabolism

Published on: May 31, 2018

8.1K
High-throughput Screening of Recalcitrance Variations in Lignocellulosic Biomass: Total Lignin, Lignin Monomers, and Enzymatic Sugar Release
11:31

High-throughput Screening of Recalcitrance Variations in Lignocellulosic Biomass: Total Lignin, Lignin Monomers, and Enzymatic Sugar Release

Published on: September 15, 2015

10.6K
Measuring Carbon-based Contaminant Mineralization Using Combined CO2 Flux and Radiocarbon Analyses
11:19

Measuring Carbon-based Contaminant Mineralization Using Combined CO2 Flux and Radiocarbon Analyses

Published on: October 21, 2016

12.5K

Area of Science:

  • Environmental Science
  • Biochemistry
  • Ecology

Background:

  • Metabolic activity in terrestrial samples is crucial for understanding ecological processes.
  • Simultaneous measurement of heat, oxygen, and carbon dioxide production offers a comprehensive view of metabolism.
  • Existing methods may have limitations in analyzing small, diverse terrestrial samples.

Purpose of the Study:

  • To develop a new vessel for simultaneous isothermal calorimetry and respirometry.
  • To enable the study of small terrestrial biological samples (<1 g).
  • To assess changes in metabolic activity over time.

Main Methods:

  • A novel vessel design for simultaneous isothermal calorimetry and respirometry (calorespirometry).
  • Utilizes a carbon dioxide absorbent within the sample vial.
  • Enables measurement of heat production rate, oxygen consumption, and carbon dioxide production.

Main Results:

  • Demonstrated the capability to study small terrestrial samples like insects, soil, leaves, and fungi.
  • Collected data on metabolic activity changes over a typical 7-hour measurement period.
  • Obtained results from experiments on leaves, a cut vegetable, and mold.

Conclusions:

  • The developed calorespirometry vessel is effective for analyzing metabolic activity in small terrestrial samples.
  • Further improvements are needed, particularly for reducing uncertainties in carbon dioxide production measurements.
  • The technique provides valuable data for ecological and biochemical research.